AJP - Endo Add DOIs to your references at manuscript stage!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 279: E997-E1002, 2000;
0193-1849/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (15)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gleason, C. E.
Right arrow Articles by Robertson, R. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gleason, C. E.
Right arrow Articles by Robertson, R. P.
Vol. 279, Issue 5, E997-E1002, November 2000

Determinants of glucose toxicity and its reversibility in the pancreatic islet beta -cell line, HIT-T15

Catherine E. Gleason, Michael Gonzalez, Jamie S. Harmon, and R. Paul Robertson

Pacific Northwest Research Institute, and Departments of Pharmacology and Medicine, University of Washington, Seattle, Washington 98122

HIT-T15 cells, a clonal beta -cell line, were cultured and passaged weekly for 6 mo in RPMI 1640 media containing various concentrations of glucose. Insulin content decreased in the intermediate- and late-passage cells as a continuous rather than a threshold glucose concentration effect. In a second series of experiments, cells were grown in media containing either 0.8 or 16.0 mM glucose from passages 76 through 105. Subcultures of passages 86, 92, and 99 that had been grown in media containing 16.0 mM glucose were switched to media containing 0.8 mM glucose and also carried forward to passage 105. Dramatic increases in insulin content and secretion and insulin gene expression were observed when the switches were made at passages 86 and 92 but not when the switch was made at passage 99. These findings suggest that glucose toxicity of insulin-secreting cells is a continuous rather than a threshold function of glucose concentration and that the shorter the period of antecedent glucose toxicity, the more likely that full recovery of cell function will occur.

insulin gene expression; PDX-1 binding


This article has been cited by other articles:


Home page
 Diabetes CareHome page
L. F. Meneghini
Early Insulin Treatment in Type 2 Diabetes: What are the pros?
Diabetes Care, November 1, 2009; 32(suppl_2): S266 - S269.
[Full Text] [PDF]

Home page
 DiabetesHome page
C. Tang, P. Han, A. I. Oprescu, S. C. Lee, A. V. Gyulkhandanyan, G. N.Y. Chan, M. B. Wheeler, and A. Giacca
Evidence for a Role of Superoxide Generation in Glucose-Induced {beta}-Cell Dysfunction In Vivo
Diabetes, November 1, 2007; 56(11): 2722 - 2731.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
D. Yin, J. Tao, D. D. Lee, J. Shen, M. Hara, J. Lopez, A. Kuznetsov, L. H. Philipson, and A. S. Chong
Recovery of Islet {beta}-Cell Function in Streptozotocin- Induced Diabetic Mice: An Indirect Role for the Spleen
Diabetes, December 1, 2006; 55(12): 3256 - 3263.
[Abstract] [Full Text] [PDF]

Home page
 Diabetes CareHome page
Y. Li, W. Xu, Z. Liao, B. Yao, X. Chen, Z. Huang, G. Hu, and J. Weng
Induction of Long-term Glycemic Control in Newly Diagnosed Type 2 Diabetic Patients Is Associated With Improvement of {beta}-Cell Function
Diabetes Care, November 1, 2004; 27(11): 2597 - 2602.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
R. P. Robertson, J. Harmon, P. O. Tran, Y. Tanaka, and H. Takahashi
Glucose Toxicity in {beta}-Cells: Type 2 Diabetes, Good Radicals Gone Bad, and the Glutathione Connection
Diabetes, March 1, 2003; 52(3): 581 - 587.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
C. S. Lee, N. J. Sund, M. Z. Vatamaniuk, F. M. Matschinsky, D. A. Stoffers, and K. H. Kaestner
Foxa2 Controls Pdx1 Gene Expression in Pancreatic {beta}-Cells In Vivo
Diabetes, August 1, 2002; 51(8): 2546 - 2551.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online