AJP - Endo Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 279: E1029-E1038, 2000;
0193-1849/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (17)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lang, C. H.
Right arrow Articles by Vary, T. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lang, C. H.
Right arrow Articles by Vary, T. C.
Vol. 279, Issue 5, E1029-E1038, November 2000

Impaired myocardial protein synthesis induced by acute alcohol intoxication is associated with changes in eIF4F

Charles H. Lang, Robert A. Frost, Vinayshree Kumar, and Thomas C. Vary

Departments of Cellular and Molecular Physiology and Surgery, Pennsylvania State University College of Medicine, Hershey, Pennsylvania 17033

The purpose of the present study was to examine potential mechanisms for the known inhibitory effect of acute alcohol exposure on myocardial protein synthesis. Rats were injected intraperitoneally with either ethanol (75 mmol/kg) or saline, and protein synthesis was measured in vivo 2.5 h thereafter by use of the flooding-dose L-[3H]phenylalanine technique. Rates of myocardial protein synthesis and translational efficiency in alcohol-treated rats were decreased compared with control values. Free (nonpolysome bound) 40S and 60S ribosomal subunits were increased 50% after alcohol treatment, indicating an impaired peptide-chain initiation. To identify mechanisms responsible for this impairment, several eukaryotic initiation factors (eIF) were analyzed. Acute alcohol intoxication did not significantly alter the myocardial content of eIF2alpha or eIF2Bvarepsilon , the extent of eIF2alpha phosphorylation, or the activity of eIF2B. Acute alcohol exposure increased the binding of 4E-binding protein 1 (4E-BP1) to eIF4E (55%), diminished the amount of eIF4E bound to eIF4G (70%), reduced the amount of 4E-BP1 in the phosphorylated gamma -form (40%), and decreased the phosphorylation of p70S6 kinase and the ribosomal protein S6. There was no significant difference in either the plasma insulin-like growth factor (IGF) I concentration (total or free) or expression of IGF-I or IGF-II mRNA in heart between the two groups. These data suggest that the acute alcohol-induced impairment in myocardial protein synthesis results, in part, from an inhibition in peptide-chain initiation, which is associated with marked changes in eIF4E availability and p70S6 kinase phosphorylation but is independent of changes in the eIF2/2B system and IGFs.

cardiomyopathy; peptide-chain initiation; eukaryotic initiation factor 4E; 4E-binding protein 1; eukaryotic initiation factor 4G


This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
T. C. Vary, S. R. Kimball, and A. Sumner
Sex-dependent differences in the regulation of myocardial protein synthesis following long-term ethanol consumption
Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2007; 292(2): R778 - R787.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
T. C. Vary, G. Deiter, and S. A. Goodman
Acute alcohol intoxication enhances myocardial eIF4G phosphorylation despite reducing mTOR signaling
Am J Physiol Heart Circ Physiol, January 1, 2005; 288(1): H121 - H128.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
C. H. Lang, R. A. Frost, and T. C. Vary
Thermal injury impairs cardiac protein synthesis and is associated with alterations in translation initiation
Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2004; 286(4): R740 - R750.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
C. J. Lynch, B. J. Patson, J. Anthony, A. Vaval, L. S. Jefferson, and T. C. Vary
Leucine is a direct-acting nutrient signal that regulates protein synthesis in adipose tissue
Am J Physiol Endocrinol Metab, September 1, 2002; 283(3): E503 - E513.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
T. C. Vary, C. J. Lynch, and C. H. Lang
Effects of chronic alcohol consumption on regulation of myocardial protein synthesis
Am J Physiol Heart Circ Physiol, September 1, 2001; 281(3): H1242 - H1251.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online