AJP - Endo Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 279: E830-E837, 2000;
0193-1849/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (6)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ryan, M. J.
Right arrow Articles by Hajduczok, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ryan, M. J.
Right arrow Articles by Hajduczok, G.
Vol. 279, Issue 4, E830-E837, October 2000

Cyclic mechanical distension regulates renin gene transcription in As4.1 cells

Michael J. Ryan1, Thomas A. Black2, Kenneth W. Gross2, and George Hajduczok1

1 Department of Physiology and Biophysics, State University of New York at Buffalo, Buffalo 14214; and 2 Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, New York 14263

The renin-producing and -secreting juxtaglomerular (JG) cells are thought to function as the baroreceptor of the kidney. The mechanism by which changes in pressure, or mechanical force, regulate renin at the molecular level has not been elucidated. The renin gene-expressing and -secreting clonal cell line As4.1 was derived from transgene-targeted oncogenesis in mice and was used as a cellular model for JG cells. As4.1 cells subjected to cyclic mechanical distension for a period of 24 h at various frequencies (0.05 or 0.5 Hz) and magnitudes (12 or 24% elongation) were analyzed via Northern analysis for renin mRNA levels. Results indicate that renin gene expression is decreased by 50-85% and returns to basal levels after a 24-h recovery period. Renin gene expression was attenuated independently of elevated cell growth or changes in renin message decay, suggesting that renin gene transcription is directly modulated by mechanical distension. Transient transfection of As4.1 cells with renin 5' flanking sequence-luciferase reporter gene constructs confirmed the role of mechanical stimulation in regulating renin gene transcription. A 43% inhibition of luciferase activity, by stretch, was observed in cells transfected with a 4,000 base pair 5' flanking sequence to the renin proximal promoter. These results demonstrate for the first time that changes in mechanical force can result in the regulation of renin gene transcription and thus provide further insight into the baroreceptor properties of renin-expressing cells.

pressure; expression; transcription; messenger ribonucleic acid stability


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
L. Pan, Y. Wang, C. A. Jones, S. T. Glenn, H. Baumann, and K. W. Gross
Enhancer-dependent inhibition of mouse renin transcription by inflammatory cytokines
Am J Physiol Renal Physiol, January 1, 2005; 288(1): F117 - F124.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
L. Pan and K. W. Gross
Transcriptional Regulation of Renin: An Update
Hypertension, January 1, 2005; 45(1): 3 - 8.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. J. Ryan, T. A. Black, S. L. Millard, K. W. Gross, and G. Hajduczok
Endothelin-1 increases calcium and attenuates renin gene expression in As4.1 cells
Am J Physiol Heart Circ Physiol, December 1, 2002; 283(6): H2458 - H2465.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
M. J. Ryan, K. W. Gross, and G. Hajduczok
Calcium-dependent activation of phospholipase C by mechanical distension in renin-expressing As4.1 cells
Am J Physiol Endocrinol Metab, October 1, 2000; 279(4): E823 - E829.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online