AJP - Endo AJP: Regulatory, Integrative and Comparative Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 279: E769-E772, 2000;
0193-1849/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (26)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rillema, J. A.
Right arrow Articles by Jhiang, S. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rillema, J. A.
Right arrow Articles by Jhiang, S. M.
Vol. 279, Issue 4, E769-E772, October 2000

Effect of prolactin on sodium iodide symporter expression in mouse mammary gland explants

James A. Rillema1, Ting Xi Yu1, and Sissy M. Jhiang2

1 Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan 48201; and 2 Department of Physiology and Cell Biology, Ohio State University, Columbus, Ohio 43210

Iodide accumulates in milk at a concentration that is more than an order of magnitude higher than the iodide concentration in maternal plasma. In earlier studies from our laboratory, we have shown that prolactin (PRL) enhances iodide accumulation by two- to threefold in cultured mammary tissues taken from pregnant mice. In the present studies, we demonstrate via Western blotting techniques that prolactin elevates the quantity of the sodium iodide symporter (NIS) in cultured mouse mammary tissues. In time-course studies, the onset of the PRL effect of NIS accumulation was found to be between 4 and 16 h after addition of PRL to the explants. The lowest PRL concentration that elicited a significant response was 1 ng/ml, and a maximum effect was elicited with PRL concentrations >100 ng/ml. Actinomycin D, cycloheximide, and thiocyanate abolished the PRL effect on NIS accumulation, whereas perchlorate was without effect. These studies suggest that the PRL stimulation of iodide accumulation in milk is mediated, at least in part, by the PRL stimulation of NIS accumulation in mammary gland tissues. These studies further demonstrate that the PRL effect on NIS accumulation occurs via an RNA protein synthesis-dependent mechanism.

iodide transporter


This article has been cited by other articles:


Home page
 J Mol EndocrinolHome page
A. J Brennan, J. A Sharp, C. M Lefevre, and K. R Nicholas
Uncoupling the mechanisms that facilitate cell survival in hormone-deprived bovine mammary explants
J. Mol. Endocrinol., September 1, 2008; 41(3): 103 - 116.
[Abstract] [Full Text] [PDF]

Home page
 Eur J EndocrinolHome page
G. Riesco-Eizaguirre and P. Santisteban
A perspective view of sodium iodide symporter research and its clinical implications.
Eur. J. Endocrinol., October 1, 2006; 155(4): 495 - 512.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
O. Dohan, A. De la Vieja, V. Paroder, C. Riedel, M. Artani, M. Reed, C. S. Ginter, and N. Carrasco
The Sodium/Iodide Symporter (NIS): Characterization, Regulation, and Medical Significance
Endocr. Rev., February 1, 2003; 24(1): 48 - 77.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. A. Rillema and M. A. Hill
Prolactin regulation of the pendrin-iodide transporter in the mammary gland
Am J Physiol Endocrinol Metab, January 1, 2003; 284(1): E25 - E28.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
J. A. Rillema, C. H. Williams, J. Moulden, and K. L. Golden
Effect of Insulin on Iodide Uptake in Mouse Mammary Gland Explants
Experimental Biology and Medicine, January 1, 2002; 227(1): 32 - 35.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online