| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Diabetes Unit, Section of Endocrinology and Departments of Medicine and Physiology, Boston Medical Center, Boston 02118; and 2 Department of Biochemistry and Molecular Biology, University of Massachusetts, Worcester, Massachusetts 01655
Malonyl-CoA acutely regulates fatty acid oxidation in liver in vivo by inhibiting carnitine palmitoyltransferase. Thus rapid increases in the concentration of malonyl-CoA, accompanied by decreases in long-chain fatty acyl carnitine (LCFA-carnitine) and fatty acid oxidation have been observed in liver of fasted-refed rats. It is less clear that it plays a similar role in skeletal muscle. To examine this question, whole body respiratory quotients (RQ) and the concentrations of malonyl-CoA and LCFA-carnitine in muscle were determined in 48-h-starved rats before and at various times after refeeding. RQ values were 0.82 at baseline and increased to 0.93, 1.0, 1.05, and 1.09 after 1, 3, 12, and 18 h of refeeding, respectively, suggesting inhibition of fat oxidation in all tissues. The increases in RQ at each time point correlated closely (r = 0.98) with increases (50-250%) in the concentration of malonyl-CoA in soleus and gastrocnemius muscles and decreases in plasma FFA and muscle LCFA-carnitine levels. Similar changes in malonyl-CoA and LCFA-carnitine were observed in liver. The increases in malonyl-CoA in muscle during refeeding were not associated with increases in the assayable activity of acetyl-CoA carboxylase (ACC) or decreases in the activity of malonyl-CoA decarboxylase (MCD). The results suggest that, during refeeding after a fast, decreases in fatty acid oxidation occur rapidly in muscle and are attributable both to decreases in plasma FFA and increases in the concentration of malonyl-CoA. They also suggest that the increase in malonyl-CoA in this situation is not due to changes in the assayable activity of either ACC or MCD or an increase in the cytosolic concentration of citrate.
acetyl-CoA carboxylase; insulin; glucose; long-chain fatty acyl carnitine; starvation; refeeding
This article has been cited by other articles:
|
|
 |
|
  E. Chalhoub, R. W. Hanson, and J. M. Belovich A computer model of gluconeogenesis and lipid metabolism in the perfused liver Am J Physiol Endocrinol Metab, December 1, 2007; 293(6): E1676 - E1686. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. S. MacLean, J. A. Higgins, M. R. Jackman, G. C. Johnson, B. K. Fleming-Elder, H. R. Wyatt, E. L. Melanson, and J. O. Hill Peripheral metabolic responses to prolonged weight reduction that promote rapid, efficient regain in obesity-prone rats Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2006; 290(6): R1577 - R1588. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Beha, H.-P. Juretschke, J. Kuhlmann, C. Neumann-Haefelin, U. Belz, M. Gerl, W. Kramer, M. Roden, and A. W. Herling Muscle type-specific fatty acid metabolism in insulin resistance: an integrated in vivo study in Zucker diabetic fatty rats Am J Physiol Endocrinol Metab, May 1, 2006; 290(5): E989 - E997. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Fediuc, M. P. Gaidhu, and R. B. Ceddia Regulation of AMP-activated protein kinase and acetyl-CoA carboxylase phosphorylation by palmitate in skeletal muscle cells J. Lipid Res., February 1, 2006; 47(2): 412 - 420. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Kiens Skeletal Muscle Lipid Metabolism in Exercise and Insulin Resistance Physiol Rev, January 1, 2006; 86(1): 205 - 243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Assifi, G. Suchankova, S. Constant, M. Prentki, A. K. Saha, and N. B. Ruderman AMP-activated protein kinase and coordination of hepatic fatty acid metabolism of starved/carbohydrate-refed rats Am J Physiol Endocrinol Metab, November 1, 2005; 289(5): E794 - E800. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. S. MacLean A peripheral perspective of weight regain Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2005; 288(6): R1447 - R1449. [Full Text] [PDF]
|
|
|
|
|
|
S. R. Commerford, L. Peng, J. J. Dube, and R. M. O'Doherty In vivo regulation of SREBP-1c in skeletal muscle: effects of nutritional status, glucose, insulin, and leptin Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2004; 287(1): R218 - R227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. P. Wojtaszewski, C. MacDonald, J. N. Nielsen, Y. Hellsten, D. G. Hardie, B. E. Kemp, B. Kiens, and E. A. Richter Regulation of 5'AMP-activated protein kinase activity and substrate utilization in exercising human skeletal muscle Am J Physiol Endocrinol Metab, April 1, 2003; 284(4): E813 - E822. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Park, V. K. Kaushik, S. Constant, M. Prentki, E. Przybytkowski, N. B. Ruderman, and A. K. Saha Coordinate Regulation of Malonyl-CoA Decarboxylase, sn-Glycerol-3-phosphate Acyltransferase, and Acetyl-CoA Carboxylase by AMP-activated Protein Kinase in Rat Tissues in Response to Exercise J. Biol. Chem., August 30, 2002; 277(36): 32571 - 32577. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. D. CLARKE, D. GASPERIKOVA, C. NELSON, A. LAPILLONNE, and W. C. HEIRD Fatty Acid Regulation of Gene Expression: A Genomic Explanation for the Benefits of the Mediterranean Diet Ann. N.Y. Acad. Sci., June 1, 2002; 967(1): 283 - 298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. K. Kaushik, M. E. Young, D. J. Dean, T. G. Kurowski, A. K. Saha, and N. B. Ruderman Regulation of fatty acid oxidation and glucose metabolism in rat soleus muscle: effects of AICAR Am J Physiol Endocrinol Metab, August 1, 2001; 281(2): E335 - E340. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T.-S. TSAO, J. LI, K. S. CHANG, A. E. STENBIT, D. GALUSKA, J. E. ANDERSON, J. R. ZIERATH, R. J. MCCARTER, and M. J. CHARRON Metabolic adaptations in skeletal muscle overexpressing GLUT4: effects on muscle and physical activity FASEB J, April 1, 2001; 15(6): 958 - 969. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Saha, A. J. Schwarsin, R. Roduit, F. Masse, V. Kaushik, K. Tornheim, M. Prentki, and N. B. Ruderman Activation of Malonyl-CoA Decarboxylase in Rat Skeletal Muscle by Contraction and the AMP-activated Protein Kinase Activator 5-Aminoimidazole-4-carboxamide-1-beta -D-ribofuranoside J. Biol. Chem., August 4, 2000; 275(32): 24279 - 24283. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-Y. Kim, T. R. Koves, G.-S. Yu, T. Gulick, R. N. Cortright, G. L. Dohm, and D. M. Muoio Evidence of a malonyl-CoA-insensitive carnitine palmitoyltransferase I activity in red skeletal muscle Am J Physiol Endocrinol Metab, May 1, 2002; 282(5): E1014 - E1022. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
