| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Hypertension and Vascular Research Division, Henry Ford Hospital, Detroit, Michigan 48202
Brain
natriuretic peptide (BNP) gene expression and chronic activation of the
sympathetic nervous system are characteristics of the development of
heart failure. We studied the role of the 
-adrenergic signaling
pathway in regulation of the human BNP (hBNP) promoter. An hBNP
promoter (
1818 to +100) coupled to a luciferase reporter gene
was transferred into neonatal cardiac myocytes, and luciferase activity
was measured as an index of promoter activity. Isoproterenol (ISO),
forskolin, and cAMP stimulated the promoter, and the
2-antagonist ICI 118,551 abrogated the effect of ISO. In
contrast, the protein kinase A (PKA) inhibitor H-89 failed to block the
action of cAMP and ISO. Pertussis toxin (PT), which inactivates
G
i, inhibited ISO- and cAMP-stimulated hBNP promoter
activity. The Src tyrosine kinase inhibitor PP1 and a dominant-negative
mutant of the small G protein Rac also abolished the effect of ISO and
cAMP. Finally, we studied the involvement of M-CAT-like binding sites
in basal and inducible regulation of the hBNP promoter. Mutation of
these elements decreased basal and cAMP-induced activity. These data
suggest that -adrenergic regulation of hBNP is PKA independent,
involves a Gi-activated pathway, and targets regulatory
elements in the proximal BNP promoter.
cardiomyocytes; gene regulation; adrenergic signaling; M-CAT elements; protein kinase A
This article has been cited by other articles:
|
|
 |
|
  M. Metrich, A. Lucas, M. Gastineau, J.-L. Samuel, C. Heymes, E. Morel, and F. Lezoualc'h Epac Mediates {beta}-Adrenergic Receptor-Induced Cardiomyocyte Hypertrophy Circ. Res., April 25, 2008; 102(8): 959 - 965. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-Y. Qian, A. Leung, P. Harding, and M. C. LaPointe PGE2 stimulates human brain natriuretic peptide expression via EP4 and p42/44 MAPK Am J Physiol Heart Circ Physiol, May 1, 2006; 290(5): H1740 - H1746. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mendez and M. C. LaPointe PGE2-induced hypertrophy of cardiac myocytes involves EP4 receptor-dependent activation of p42/44 MAPK and EGFR transactivation Am J Physiol Heart Circ Physiol, May 1, 2005; 288(5): H2111 - H2117. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Goetze, W. Yongzhong, J. F Rehfeld, E. Jorgensen, and J. Kastrup Coronary angiography transiently increases plasma pro-B-type natriuretic peptide Eur. Heart J., May 1, 2004; 25(9): 759 - 764. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Mendez and M. C. LaPointe PPAR{gamma} Inhibition of Cyclooxygenase-2, PGE2 Synthase, and Inducible Nitric Oxide Synthase in Cardiac Myocytes Hypertension, October 1, 2003; 42(4): 844 - 850. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. LaPointe, X.-P. Yang, O. A. Carretero, and Q. He Left ventricular targeting of reporter gene expression in vivo by human BNP promoter in an adenoviral vector Am J Physiol Heart Circ Physiol, October 1, 2002; 283(4): H1439 - H1445. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. He, M. Mendez, and M. C. LaPointe Regulation of the human brain natriuretic peptide gene by GATA-4 Am J Physiol Endocrinol Metab, July 1, 2002; 283(1): E50 - E57. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. C Kone Molecular biology of natriuretic peptides and nitric oxide synthases Cardiovasc Res, August 15, 2001; 51(3): 429 - 441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. He, D. Wang, X.-P. Yang, O. A. Carretero, and M. C. LaPointe Inducible regulation of human brain natriuretic peptide promoter in transgenic mice Am J Physiol Heart Circ Physiol, January 1, 2001; 280(1): H368 - H376. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
