| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 The John B. Pierce Laboratory, 2 Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06519
Fasting elicits a progressive increase in lipid metabolism within skeletal muscle. To determine the effects of fasting on the transcriptional regulation of genes important for metabolic control in skeletal muscle composed of different fiber types, nuclei from control and fasted (24 and 72 h) rats were subjected to nuclear run-on analysis using an RT-PCR-based technique. Fasting increased (P < 0.05) transcription rate of the muscle-specific uncoupling protein-3 gene (UCP3) 14.3- to 21.1-fold in white gastrocnemius (WG; fast-twitch glycolytic) and 5.5- to 7.5-fold in red gastrocnemius (RG; fast-twitch oxidative) and plantaris (PL; mixed) muscles. No change occurred in soleus (slow-twitch oxidative) muscle. Fasting also increased transcription rate of the lipoprotein lipase (LPL), muscle carnitine palmitoyltransferase I (CPT I), and long-chain acyl-CoA dehydrogenase (LCAD) genes 1.7- to 3.7-fold in WG, RG, and PL muscles. Transcription rate responses were similar after 24 and 72 h of fasting. Surprisingly, increasing metabolic demand during the initial 8 h of starvation (two 2-h bouts of treadmill running) attenuated the 24-h fasting-induced transcriptional activation of UCP3, LPL, CPT I, and LCAD in RG and PL muscles, suggesting the presence of opposing regulatory mechanisms. These data demonstrate that fasting elicits a fiber type-specific coordinate increase in the transcription rate of several genes involved in and/or required for lipid metabolism and indicate that exercise may attenuate the fasting-induced transcriptional activation of specific metabolic genes.
starvation; metabolism; medium-chain acyl-coenzyme A dehydrogenase; hexokinase II; exercise
This article has been cited by other articles:
|
|
 |
|
  V. Kolla, L. W. Gonzales, N. A. Bailey, P. Wang, S. Angampalli, M. H. Godinez, M. Madesh, and P. L. Ballard Carcinoembryonic cell adhesion molecule 6 in human lung: regulated expression of a multifunctional type II cell protein Am J Physiol Lung Cell Mol Physiol, June 1, 2009; 296(6): L1019 - L1030. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Zwetsloot, L. M. Westerkamp, B. F. Holmes, and T. P. Gavin AMPK regulates basal skeletal muscle capillarization and VEGF expression, but is not necessary for the angiogenic response to exercise J. Physiol., December 15, 2008; 586(24): 6021 - 6035. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. C. Long and J. R. Zierath Influence of AMP-activated protein kinase and calcineurin on metabolic networks in skeletal muscle Am J Physiol Endocrinol Metab, September 1, 2008; 295(3): E545 - E552. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. L. Gouill, M. Jimenez, C. Binnert, P.-Y. Jayet, S. Thalmann, P. Nicod, U. Scherrer, and P. Vollenweider Endothelial Nitric Oxide Synthase (eNOS) Knockout Mice Have Defective Mitochondrial {beta}-Oxidation Diabetes, November 1, 2007; 56(11): 2690 - 2696. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. B. Clizbe, M. L. Owens, K. R. Masuda, J. E. Shackelford, and S. K. Krisans IDI2, a Second Isopentenyl Diphosphate Isomerase in Mammals J. Biol. Chem., March 2, 2007; 282(9): 6668 - 6676. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Higami, T. Tsuchiya, T. Chiba, H. Yamaza, I. Muraoka, M. Hirose, T. Komatsu, and I. Shimokawa Hepatic Gene Expression Profile of Lipid Metabolism in Rats: Impact of Caloric Restriction and Growth Hormone/Insulin-Like Growth Factor-1 Suppression J. Gerontol. A Biol. Sci. Med. Sci., November 1, 2006; 61(11): 1099 - 1110. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. L. Sensky, K. K. Jewell, K. J. P. Ryan, T. Parr, R. G. Bardsley, and P. J. Buttery Effect of anabolic agents on calpastatin promoters in porcine skeletal muscle and their responsiveness to cyclic adenosine monophosphate-and calcium-related stimuli J Anim Sci, November 1, 2006; 84(11): 2973 - 2982. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Civitarese, M. K. C. Hesselink, A. P. Russell, E. Ravussin, and P. Schrauwen Glucose ingestion during exercise blunts exercise-induced gene expression of skeletal muscle fat oxidative genes Am J Physiol Endocrinol Metab, December 1, 2005; 289(6): E1023 - E1029. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. B. PATEL, S. WORRALL, P. W. EMERY, and V. R. PREEDY PROTEIN ADDUCT SPECIES IN MUSCLE AND LIVER OF RATS FOLLOWING ACUTE ETHANOL ADMINISTRATION Alcohol Alcohol., November 1, 2005; 40(6): 485 - 493. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K De Bock, E. A Richter, A. P Russell, B. O Eijnde, W Derave, M Ramaekers, E Koninckx, B Leger, J Verhaeghe, and P Hespel Exercise in the fasted state facilitates fibre type-specific intramyocellular lipid breakdown and stimulates glycogen resynthesis in humans J. Physiol., April 15, 2005; 564(2): 649 - 660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Akerstrom, A. Steensberg, P. Keller, C. Keller, M. Penkowa, and B. K. Pedersen Exercise induces interleukin-8 expression in human skeletal muscle J. Physiol., March 1, 2005; 563(2): 507 - 516. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Gealy, M. Denson, C. Humphreys, B. McSharry, G. Wilkinson, and R. Caswell Posttranscriptional Suppression of Interleukin-6 Production by Human Cytomegalovirus J. Virol., January 1, 2005; 79(1): 472 - 485. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Hildebrandt, H. Pilegaard, and P. D. Neufer Differential transcriptional activation of select metabolic genes in response to variations in exercise intensity and duration Am J Physiol Endocrinol Metab, November 1, 2003; 285(5): E1021 - E1027. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Schmitt, M. Fluck, J. Decombaz, R. Kreis, C. Boesch, M. Wittwer, F. Graber, M. Vogt, H. Howald, and H. Hoppeler Transcriptional adaptations of lipid metabolism in tibialis anterior muscle of endurance-trained athletes Physiol Genomics, October 17, 2003; 15(2): 148 - 157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Sun, C. Wray, X. Tian, P.-O. Hasselgren, and J. Lu Expression of uncoupling protein 3 is upregulated in skeletal muscle during sepsis Am J Physiol Endocrinol Metab, September 1, 2003; 285(3): E512 - E520. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Haddad, R. R. Roy, H. Zhong, V. R. Edgerton, and K. M. Baldwin Atrophy responses to muscle inactivity. II. Molecular markers of protein deficits J Appl Physiol, August 1, 2003; 95(2): 791 - 802. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Pilegaard, B. Saltin, and P. D. Neufer Effect of Short-Term Fasting and Refeeding on Transcriptional Regulation of Metabolic Genes in Human Skeletal Muscle Diabetes, March 1, 2003; 52(3): 657 - 662. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Huey, F. Haddad, A. X. Qin, and K. M. Baldwin Transcriptional regulation of the type I myosin heavy chain gene in denervated rat soleus Am J Physiol Cell Physiol, March 1, 2003; 284(3): C738 - C748. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Cameron-Smith, L. M Burke, D. J Angus, R. J Tunstall, G. R Cox, A. Bonen, J. A Hawley, and M. Hargreaves A short-term, high-fat diet up-regulates lipid metabolism and gene expression in human skeletal muscle Am. J. Clinical Nutrition, February 1, 2003; 77(2): 313 - 318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Pilegaard, B. Saltin, and P D. Neufer Exercise induces transient transcriptional activation of the PGC-1{alpha} gene in human skeletal muscle J. Physiol., February 1, 2003; 546(3): 851 - 858. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Stoppani, A. L. Hildebrandt, K. Sakamoto, D. Cameron-Smith, L. J. Goodyear, and P. D. Neufer AMP-activated protein kinase activates transcription of the UCP3 and HKII genes in rat skeletal muscle Am J Physiol Endocrinol Metab, December 1, 2002; 283(6): E1239 - E1248. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. T. JAGOE, S. H. LECKER, M. GOMES, and A. L. GOLDBERG Patterns of gene expression in atrophying skeletal muscles: response to food deprivation FASEB J, November 1, 2002; 16(13): 1697 - 1712. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Pilegaard, C. Keller, A. Steensberg, J. Wulff Helge, B. Klarlund Pedersen, B. Saltin, and P D. Neufer Influence of pre-exercise muscle glycogen content on exercise-induced transcriptional regulation of metabolic genes J. Physiol., May 15, 2002; 541(1): 261 - 271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Argyropoulos and M.-E. Harper Molecular Biology of Thermoregulation: Invited Review: Uncoupling proteins and thermoregulation J Appl Physiol, May 1, 2002; 92(5): 2187 - 2198. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Himms-Hagen and M.-E. Harper Physiological Role of UCP3 May Be Export of Fatty Acids from Mitochondria When Fatty Acid Oxidation Predominates: An Hypothesis Experimental Biology and Medicine, February 1, 2001; 226(2): 78 - 84. [Abstract] [Full Text]
|
|
|
|
|
|
H. Pilegaard, G. A. Ordway, B. Saltin, and P. D. Neufer Transcriptional regulation of gene expression in human skeletal muscle during recovery from exercise Am J Physiol Endocrinol Metab, October 1, 2000; 279(4): E806 - E814. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
