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Am J Physiol Endocrinol Metab 278: E285-E292, 2000;
0193-1849/00 $5.00
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Vol. 278, Issue 2, E285-E292, February 2000

Novel salmon cardiac peptide hormone is released from the ventricle by regulated secretory pathway

Kati Kokkonen1, Heidi Vierimaa1, Sari Bergström1, Virpi Tervonen1, Olli Arjamaa2, Heikki Ruskoaho3, Matti Järvilehto4, and Olli Vuolteenaho1

Departments of 1 Physiology and 3 Pharmacology and Toxicology, Biocenter Oulu, 2 Institute of Arctic Medicine, 4 Department of Biology, University of Oulu, FIN-90014 Oulu, Finland

We used the secretion of the novel salmon cardiac peptide (sCP) as a model to examine the mechanisms of ventricular hormone release. Mechanical load increased dose dependently the secretion of immunoreactive sCP from isolated perfused salmon ventricle, with 3.3-fold increase when a load of 13 cmH2O was applied. Endothelin-1 (5 nmol/l) was also able to rapidly increase the secretion of sCP. The released peptide corresponded to the biologically active sCP-29, whereas the large ventricular storage consisted of pro-sCP-sized material. With the use of immunoelectron microscopy, a large number of granules containing immunoreactive sCP could be detected in salmon ventricle. As judged by RNA blot analysis, there was very active basal expression of the sCP gene in the ventricle, which was not increased by mechanical load of up to 2-h duration. Our results show that the ventricle actively expresses the gene of sCP, stores the prohormone in secretory granules, and releases the peptide in response to mechanical load and endothelin-1. Thus the salmon ventricle uses the regulated pathway to produce and release a hormone structurally related to the mammalian natriuretic peptides.

natriuretic peptide; mechanical load; osmoregulation; posttranslational processing; endothelin


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