AJP - Endo Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 277: E1142-E1149, 1999;
0193-1849/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gelardi, N. L.
Right arrow Articles by Cowett, R. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gelardi, N. L.
Right arrow Articles by Cowett, R. M.
Vol. 277, Issue 6, E1142-E1149, December 1999

Insulin resistance and glucose transporter expression during the euglycemic hyperinsulinemic clamp in the lamb

Nancy L. Gelardi1,2, Robert E. Rapoza1, Joseph F. Renzulli1, and Richard M. Cowett1,2,3

1 Department of Pediatrics, Women & Infants Hospital, Brown University School of Medicine, Providence, Rhode Island 02905-2401; 2 Robert Schwartz MD Center for Metabolism and Nutrition, and the Division of Neonatology, MetroHealth Medical Center, Cleveland 44109-1998; and 3 Department of Neonatology, The Children's Hospital, Cleveland Clinic Foundation, Cleveland, Ohio 44195-0001

Three- to six-day-old lambs infused with 100 mU · kg-1 · min-1 insulin required greater amounts of glucose to maintain euglycemia during a euglycemic hyperinsulinemic clamp compared with 31- to 35-day-old insulin-infused lambs (15.87 ± 3.47 vs. 4.30 ± 1.11 mg · kg-1 · min-1, P < 0.05, respectively). Endogenous glucose production persisted in both groups; however, the percent decrease compared with age-matched lambs receiving no insulin was greater in the younger group compared with the older group (53%, P < 0.001, vs. 34%, P < 0.01). The younger animals showed greater glucose utilization compared with the older animals (215 vs. 96%, respectively, P < 0.01). No effect of insulin was noted on GLUT-4 protein expression in either group. GLUT-2 expression was increased in older vs. younger lambs. Older insulin-infused lambs showed lower GLUT-2 expression than older 0 insulin-infused lambs [0.94 ± 0.07 vs. 1.64 ± 0.10 (OD) units, P < 0.005]. Increased sensitivity to insulin in the younger animals was not related to acute changes in GLUT-4 expression. Increased GLUT-2 expression with age, as well as decreased expression with hyperinsulinemia, is consistent with the development of an insulin-resistant state in the adult.

neonatal glucose homeostasis


This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
K. L. Gatford, M. J. De Blasio, P. Thavaneswaran, J. S. Robinson, I. C. McMillen, and J. A. Owens
Postnatal ontogeny of glucose homeostasis and insulin action in sheep
Am J Physiol Endocrinol Metab, June 1, 2004; 286(6): E1050 - E1059.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. S. Anderson, J. Flowers-Ziegler, U. G. Das, W. W. Hay Jr., and S. U. Devaskar
Glucose transporter protein responses to selective hyperglycemia or hyperinsulinemia in fetal sheep
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2001; 281(5): R1545 - R1552.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online