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Am J Physiol Endocrinol Metab 277: E1111-E1121, 1999;
0193-1849/99 $5.00
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Vol. 277, Issue 6, E1111-E1121, December 1999

Use of a single 13C NMR resonance of glutamate for measuring oxygen consumption in tissue

F. Mark H. Jeffrey1, Alexander Reshetov3, Charles J. Storey1, Rui A. Carvalho1, A. Dean Sherry1,4, and Craig R. Malloy1,2

1 Department of Radiology, The Mary Nell and Ralph B. Rogers Magnetic Resonance Center, 2 Division of Cardiology, Department of Internal Medicine, Dallas Veterans Affairs Medical Center, University of Texas Southwestern Medical Center, Dallas, Texas 75235-9085; 3 Intel, Santa Clara, California 95052-8119; and 4 Department of Chemistry, University of Texas at Dallas, Richardson, Texas 75083-0688

A kinetic model of the citric acid cycle for calculating oxygen consumption from 13C nuclear magnetic resonance (NMR) multiplet data has been developed. Measured oxygen consumption (MVO2) was compared with MVO2 predicted by the model with 13C NMR data obtained from rat hearts perfused with glucose and either [2-13C]acetate or [3-13C]pyruvate. The accuracy of MVO2 measured from three subsets of NMR data was compared: glutamate C-4 and C-3 resonance areas; the doublet C4D34 (expressed as a fraction of C-4 area); and C-4 and C-3 areas plus several multiplets of C-2, C-3, and C-4. MVO2 determined by set 2 (C4D34 only) gave the same degree of accuracy as set 3 (complete data); both were superior to set 1 (C-4 and C-3 areas). Analysis of the latter suffers from the correlation between citric acid cycle flux and exchange between alpha -ketoglutarate and glutamate, resulting in greater error in estimating MVO2. Analysis of C4D34 is less influenced by correlation between parameters, and this single measurement provides the best opportunity for a noninvasive measurement of oxygen consumption.

13C isotopomer analysis; citric acid cycle flux; heart metabolism; nuclear magnetic resonance


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