AJP - Endo AJP: Lung Cellular and Molecular Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 277: E798-E804, 1999;
0193-1849/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Liu, X.
Right arrow Articles by Dhalla, N. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liu, X.
Right arrow Articles by Dhalla, N. S.
Vol. 277, Issue 5, E798-E804, November 1999

Changes in cardiac protein kinase C activities and isozymes in streptozotocin-induced diabetes

Xueliang Liu1, Jingwei Wang1, Nobuakira Takeda2, Luciano Binaglia3, Vincenzo Panagia1, and Naranjan S. Dhalla1

1 Institute of Cardiovascular Sciences, St. Boniface General Hospital Research Centre and Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba R2H 2A6, Canada; 2 Aoto Hospital, Department of Internal Medicine, Jikei University, Tokyo 125, Japan; and 3 Institute of Biochemistry, University of Perugia, 06100 Perugia, Italy

To understand cardiac dysfunction in diabetes, the activity of protein kinase C (PKC) and protein contents of its isozymes (PKC-alpha , -beta , -epsilon , and -zeta ) were examined in diabetic rats upon injection of streptozotocin (65 mg/kg iv). The hearts were removed at 1, 2, 4, and 8 wk, and some of the 6-wk diabetic animals had been injected with insulin (3 U/day) for 2 wk. The Ca2+-dependent PKC activity was increased by 43 and 51% in the homogenate fraction and 31 and 70% in the cytosolic fraction from the 4- and 8-wk diabetic hearts, respectively, in comparison with control values. The Ca2+-independent PKC activity was increased by 24 and 32% in the homogenate fraction and 52 and 89% in the cytosolic fraction from the 4- and 8-wk diabetic hearts, respectively, in comparison with control values. The relative protein contents of PKC-alpha , -beta , -epsilon , and -zeta isozymes were increased by 43, 31, 48, and 38%, respectively, in the homogenate fraction and by 126, 119, 148, and 129%, respectively, in the cytosolic fraction of the 8-wk diabetic heart. The observed changes in heart homogenate and cytosolic fractions were partially reversible upon treatment of the diabetic rats with insulin. The results suggest that the increased myocardial PKC activity and increased protein contents of the cytosolic PKC isozymes are associated with subcellular alterations and cardiac dysfunction in the diabetic heart.

diabetic cardiomyopathy; diabetic heart dysfunction


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
P. Rodriguez, B. Mitton, P. Nicolaou, G. Chen, and E. G. Kranias
Phosphorylation of human inhibitor-1 at Ser67 and/or Thr75 attenuates stimulatory effects of protein kinase A signaling in cardiac myocytes
Am J Physiol Heart Circ Physiol, July 1, 2007; 293(1): H762 - H769.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
V. Pastukh, S. Wu, C. Ricci, M. Mozaffari, and S. Schaffer
Reversal of hyperglycemic preconditioning by angiotensin II: role of calcium transport
Am J Physiol Heart Circ Physiol, April 1, 2005; 288(4): H1965 - H1975.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. M. Tickerhoof, P A. Farrell, and D. H. Korzick
Alterations in rat coronary vasoreactivity and vascular protein kinase C isoforms in Type 1 diabetes
Am J Physiol Heart Circ Physiol, December 1, 2003; 285(6): H2694 - H2703.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. Wang, X. Liu, E. Sentex, N. Takeda, and N. S. Dhalla
Increased expression of protein kinase C isoforms in heart failure due to myocardial infarction
Am J Physiol Heart Circ Physiol, June 1, 2003; 284(6): H2277 - H2287.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
M. Guo, M. H. Wu, F. Korompai, and S. Y. Yuan
Upregulation of PKC genes and isozymes in cardiovascular tissues during early stages of experimental diabetes
Physiol Genomics, January 15, 2003; 12(2): 139 - 146.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
K. J. Way, K. Isshiki, K. Suzuma, T. Yokota, D. Zvagelsky, F. J. Schoen, G. E. Sandusky, P. A. Pechous, C. J. Vlahos, H. Wakasaki, et al.
Expression of Connective Tissue Growth Factor Is Increased in Injured Myocardium Associated With Protein Kinase C {beta}2 Activation and Diabetes
Diabetes, September 1, 2002; 51(9): 2709 - 2718.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
Y. Hiramatsu, N. Sekiguchi, M. Hayashi, K. Isshiki, T. Yokota, G. L. King, and M. R. Loeken
Diacylglycerol Production and Protein Kinase C Activity Are Increased in a Mouse Model of Diabetic Embryopathy
Diabetes, September 1, 2002; 51(9): 2804 - 2810.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
L. Laviola, G. Belsanti, A. M. Davalli, R. Napoli, S. Perrini, G. C. Weir, R. Giorgino, and F. Giorgino
Effects of Streptozocin Diabetes and Diabetes Treatment by Islet Transplantation on In Vivo Insulin Signaling in Rat Heart
Diabetes, December 1, 2001; 50(12): 2709 - 2720.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online