AJP - Endo Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 277: E733-E741, 1999;
0193-1849/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thorell, A.
Right arrow Articles by Goodyear, L. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thorell, A.
Right arrow Articles by Goodyear, L. J.
Vol. 277, Issue 4, E733-E741, October 1999

Exercise and insulin cause GLUT-4 translocation in human skeletal muscle

Anders Thorell1, Michael F. Hirshman2, Jonas Nygren3, Lennart Jorfeldt4, Jørgen F. P. Wojtaszewski2, Scott D. Dufresne2, Edward S. Horton2, Olle Ljungqvist1, and Laurie J. Goodyear2

1 Department of Surgery, Huddinge University Hospital, S-141 86 Huddinge; Departments of 3 Surgery and 4 Thoracic Clinical Physiology, Karolinska Hospital and Karolinska Institute, S-17177 Stockholm, Sweden; and 2 Joslin Diabetes Center and Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02215

Studies in rodents have established that GLUT-4 translocation is the major mechanism by which insulin and exercise increase glucose uptake in skeletal muscle. In contrast, much less is known about the translocation phenomenon in human skeletal muscle. In the current study, nine healthy volunteers were studied on two different days. On one day, biopsies of vastus lateralis muscle were taken before and after a 2-h euglycemic-hyperinsulinemic clamp (0.8 mU · kg-1 · min-1). On another day, subjects exercised for 60 min at 70% of maximal oxygen consumption (VO2 max), a biopsy was obtained, and the same clamp and biopsy procedure was performed as that during the previous experiment. Compared with insulin treatment alone, glucose infusion rates were significantly increased during the postexercise clamp for the periods 0-30 min, 30-60 min, and 60-90 min, but not during the last 30 min of the clamp. Plasma membrane GLUT-4 content was significantly increased in response to physiological hyperinsulinemia (32% above rest), exercise (35%), and the combination of exercise plus insulin (44%). Phosphorylation of Akt, a putative signaling intermediary for GLUT-4 translocation, was increased in response to insulin (640% above rest), exercise (280%), and exercise plus insulin (1,000%). These data demonstrate that two normal physiological conditions, moderate intensity exercise and physiological hyperinsulinemia ~56 µU/ml, cause GLUT-4 translocation and Akt phosphorylation in human skeletal muscle.

glucose transporters; glucose uptake; Akt; glucose disposal; muscle contraction


This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
K. F. Howlett, A. Mathews, A. Garnham, and K. Sakamoto
The effect of exercise and insulin on AS160 phosphorylation and 14-3-3 binding capacity in human skeletal muscle
Am J Physiol Endocrinol Metab, February 1, 2008; 294(2): E401 - E407.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
C. Frosig, M. P. Sajan, S. J. Maarbjerg, N. Brandt, C. Roepstorff, J. F. P. Wojtaszewski, B. Kiens, R. V. Farese, and E. A. Richter
Exercise improves phosphatidylinositol-3,4,5-trisphosphate responsiveness of atypical protein kinase C and interacts with insulin signalling to peptide elongation in human skeletal muscle
J. Physiol., August 1, 2007; 582(3): 1289 - 1301.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
G. D. Wadley, N. Konstantopoulos, L. Macaulay, K. F. Howlett, A. Garnham, M. Hargreaves, and D. Cameron-Smith
Increased insulin-stimulated Akt pSer473 and cytosolic SHP2 protein abundance in human skeletal muscle following acute exercise and short-term training
J Appl Physiol, April 1, 2007; 102(4): 1624 - 1631.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. T. Treebak, J. B. Birk, A. J. Rose, B. Kiens, E. A. Richter, and J. F. P. Wojtaszewski
AS160 phosphorylation is associated with activation of {alpha}2beta2{gamma}1- but not {alpha}2beta2{gamma}3-AMPK trimeric complex in skeletal muscle during exercise in humans
Am J Physiol Endocrinol Metab, March 1, 2007; 292(3): E715 - E722.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
T. Hamada, E. B. Arias, and G. D. Cartee
Increased submaximal insulin-stimulated glucose uptake in mouse skeletal muscle after treadmill exercise
J Appl Physiol, November 1, 2006; 101(5): 1368 - 1376.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Coll. Nutr.Home page
V. Englert, K. Wells, W. Long, M. S. Hickey, and C. L. Melby
Effect of Acute Prior Exercise on Glycemic and Insulinemic Indices
J. Am. Coll. Nutr., June 1, 2006; 25(3): 195 - 202.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Y. Li, P. Wang, J. Xu, and G. V. Desir
Voltage-gated potassium channel Kv1.3 regulates GLUT4 trafficking to the plasma membrane via a Ca2+-dependent mechanism
Am J Physiol Cell Physiol, February 1, 2006; 290(2): C345 - C351.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
A. J. Rose and E. A. Richter
Skeletal Muscle Glucose Uptake During Exercise: How is it Regulated?
Physiology, August 1, 2005; 20(4): 260 - 270.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
B. Braun, C. Sharoff, S. R. Chipkin, and F. Beaudoin
Effects of insulin resistance on substrate utilization during exercise in overweight women
J Appl Physiol, September 1, 2004; 97(3): 991 - 997.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
A. R. Gosmanov, N. C. Nordtvedt, R. Brown, and D. B. Thomason
Exercise effects on muscle beta -adrenergic signaling for MAPK-dependent NKCC activity are rapid and persistent
J Appl Physiol, October 1, 2002; 93(4): 1457 - 1465.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
K. Sakamoto and L. J. Goodyear
Exercise Effects on Muscle Insulin Signaling and Action: Invited Review: Intracellular signaling in contracting skeletal muscle
J Appl Physiol, July 1, 2002; 93(1): 369 - 383.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. F. P. Wojtaszewski, J. N. Nielsen, and E. A. Richter
Exercise Effects on Muscle Insulin Signaling and Action: Invited Review: Effect of acute exercise on insulin signaling and action in humans
J Appl Physiol, July 1, 2002; 93(1): 384 - 392.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
F. S.L. Thong, W. Derave, B. Kiens, T. E. Graham, B. Urso, J. F.P. Wojtaszewski, B. F. Hansen, and E. A. Richter
Caffeine-Induced Impairment of Insulin Action but Not Insulin Signaling in Human Skeletal Muscle Is Reduced by Exercise
Diabetes, March 1, 2002; 51(3): 583 - 590.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
R. L. P. G. Jentjens, L. J. C. van Loon, C. H. Mann, A. J. M. Wagenmakers, and A. E. Jeukendrup
Addition of protein and amino acids to carbohydrates does not enhance postexercise muscle glycogen synthesis
J Appl Physiol, August 1, 2001; 91(2): 839 - 846.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
W. Derave, B. F. Hansen, S. Lund, S. Kristiansen, and E. A. Richter
Muscle glycogen content affects insulin-stimulated glucose transport and protein kinase B activity
Am J Physiol Endocrinol Metab, November 1, 2000; 279(5): E947 - E955.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
A. M. Persky and G. A. Brazeau
Clinical Pharmacology of the Dietary Supplement Creatine Monohydrate
Pharmacol. Rev., June 1, 2001; 53(2): 161 - 176.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online