AJP - Endo Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 277: E724-E732, 1999;
0193-1849/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wojtaszewski, J. F. P.
Right arrow Articles by Richter, E. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wojtaszewski, J. F. P.
Right arrow Articles by Richter, E. A.
Vol. 277, Issue 4, E724-E732, October 1999

Differential regulation of MAP kinase by contraction and insulin in skeletal muscle: metabolic implications

Jørgen F. P. Wojtaszewski1,2, Jan Lynge1, Allan B. Jakobsen1, Laurie J. Goodyear2, and Erik A. Richter1

1 Copenhagen Muscle Research Centre, August Krogh Institute, Copenhagen University, DK-2100 Copenhagen, Denmark; and 2 Research Division, Joslin Diabetes Center, and Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02215

We have investigated the activation of the extracellular signal-regulated kinases (ERK1 and ERK2) by muscle contraction and insulin in perfused rat skeletal muscle. Both stimuli activated ERK1 and ERK2 by an upstream kinase MAP/ERK kinase (MEK)-dependent mechanism, as the MEK inhibitor PD-98059 inhibited ERK phosphorylation. The presence of the phosphatidylinositol (PI) 3-kinase inhibitors LY-294002 and wortmannin totally eradicated ERK1 and ERK2 phosphorylation in response to insulin but not contraction. Insulin and muscle contraction activated muscle glucose transport, glycogen synthase, and amino acid transport independently of ERK signaling, whereas the PI 3-kinase inhibitors abolished the stimulatory effects of insulin but not those of contraction on these three cellular processes. We conclude that 1) insulin and contraction activate ERK signaling in skeletal muscle; 2) ERK signaling is not necessary for activation of glucose and amino acid transport or glycogen synthase activity by contraction and insulin in skeletal muscle; and 3) insulin-induced activation of MEK, the upstream activator of ERK, is dependent on PI 3-kinase, whereas contraction utilizes a different mechanism.

glucose and amino acid transport; glycogen synthase; phosphatidylinositol 3-kinase; extracellular signal-regulated kinase; MEK; PD-98059; LY-294002; wortmannin


This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
S. J. Maarbjerg, S. B. Jorgensen, A. J. Rose, J. Jeppesen, T. E. Jensen, J. T. Treebak, J. B. Birk, P. Schjerling, J. F. P. Wojtaszewski, and E. A. Richter
Genetic impairment of AMPK{alpha}2 signaling does not reduce muscle glucose uptake during treadmill exercise in mice
Am J Physiol Endocrinol Metab, October 1, 2009; 297(4): E924 - E934.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
H. F. Kramer and L. J. Goodyear
Exercise, MAPK, and NF-{kappa}B signaling in skeletal muscle
J Appl Physiol, July 1, 2007; 103(1): 388 - 395.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
M. K. Todd, M. J. Watt, J. Le, A. L. Hevener, and L. P. Turcotte
Thiazolidinediones enhance skeletal muscle triacylglycerol synthesis while protecting against fatty acid-induced inflammation and insulin resistance
Am J Physiol Endocrinol Metab, February 1, 2007; 292(2): E485 - E493.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. P. Thyfault, M. G. Cree, D. Zheng, J. J. Zwetsloot, E. B. Tapscott, T. R. Koves, O. Ilkayeva, R. R. Wolfe, D. M. Muoio, and G. L. Dohm
Contraction of insulin-resistant muscle normalizes insulin action in association with increased mitochondrial activity and fatty acid catabolism
Am J Physiol Cell Physiol, February 1, 2007; 292(2): C729 - C739.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
N. Fujii, N. Jessen, and L. J. Goodyear
AMP-activated protein kinase and the regulation of glucose transport
Am J Physiol Endocrinol Metab, November 1, 2006; 291(5): E867 - E877.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
A. J. Rose and E. A. Richter
Skeletal Muscle Glucose Uptake During Exercise: How is it Regulated?
Physiology, August 1, 2005; 20(4): 260 - 270.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
N. Jessen and L. J. Goodyear
Contraction signaling to glucose transport in skeletal muscle
J Appl Physiol, July 1, 2005; 99(1): 330 - 337.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. D. Parkington, N. K. LeBrasseur, A. P. Siebert, and R. A. Fielding
Contraction-mediated mTOR, p70S6k, and ERK1/2 phosphorylation in aged skeletal muscle
J Appl Physiol, July 1, 2004; 97(1): 243 - 248.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
Y. Leng, T. L. Steiler, and J. R. Zierath
Effects of Insulin, Contraction, and Phorbol Esters on Mitogen-Activated Protein Kinase Signaling in Skeletal Muscle From Lean and ob/ob Mice
Diabetes, June 1, 2004; 53(6): 1436 - 1444.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
M. J. Watt, P. Krustrup, N. H. Secher, B. Saltin, B. K. Pedersen, and M. A. Febbraio
Glucose ingestion blunts hormone-sensitive lipase activity in contracting human skeletal muscle
Am J Physiol Endocrinol Metab, January 1, 2004; 286(1): E144 - E150.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. R. Gosmanov, E. G. Schneider, and D. B. Thomason
NKCC activity restores muscle water during hyperosmotic challenge independent of insulin, ERK, and p38 MAPK
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2003; 284(3): R655 - R665.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
A. R. Gosmanov, N. C. Nordtvedt, R. Brown, and D. B. Thomason
Exercise effects on muscle beta -adrenergic signaling for MAPK-dependent NKCC activity are rapid and persistent
J Appl Physiol, October 1, 2002; 93(4): 1457 - 1465.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
K. Sakamoto and L. J. Goodyear
Exercise Effects on Muscle Insulin Signaling and Action: Invited Review: Intracellular signaling in contracting skeletal muscle
J Appl Physiol, July 1, 2002; 93(1): 369 - 383.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. F. P. Wojtaszewski, J. N. Nielsen, and E. A. Richter
Exercise Effects on Muscle Insulin Signaling and Action: Invited Review: Effect of acute exercise on insulin signaling and action in humans
J Appl Physiol, July 1, 2002; 93(1): 384 - 392.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
R. V. Farese
Function and dysfunction of aPKC isoforms for glucose transport in insulin-sensitive and insulin-resistant states
Am J Physiol Endocrinol Metab, July 1, 2002; 283(1): E1 - E11.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. J. F. P. Luiken, D. J. Dyck, X.-X. Han, N. N. Tandon, Y. Arumugam, J. F. C. Glatz, and A. Bonen
Insulin induces the translocation of the fatty acid transporter FAT/CD36 to the plasma membrane
Am J Physiol Endocrinol Metab, February 1, 2002; 282(2): E491 - E495.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
C. J. Carlson, Z. Fan, S. E. Gordon, and F. W. Booth
Time course of the MAPK and PI3-kinase response within 24 h of skeletal muscle overload
J Appl Physiol, November 1, 2001; 91(5): 2079 - 2087.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
A. A. Osman, M. Pendergrass, J. Koval, K. Maezono, K. Cusi, T. Pratipanawatr, and L. J. Mandarino
Regulation of MAP kinase pathway activity in vivo in human skeletal muscle
Am J Physiol Endocrinol Metab, June 1, 2000; 278(6): E992 - E999.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online