AJP - Endo Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 277: E464-E473, 1999;
0193-1849/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Raina, N.
Right arrow Articles by Jeejeebhoy, K. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Raina, N.
Right arrow Articles by Jeejeebhoy, K. N.
Vol. 277, Issue 3, E464-E473, September 1999

EPILOGUE
Effect of nutrition on tumor necrosis factor receptors in weight-gaining and -losing rats

Nilima Raina1, Jonathan Lamarre2, Choong-Ching Liew1, Amir H. Lofti1, and Khursheed N. Jeejeebhoy1

1 Departments of Nutrition and Medicine, University of Toronto, Toronto, Ontario M5S 1A8; and 2 Department of Biomedical Sciences, University of Guelph, Guelph, Ontario, Canada N1G 2W1

Previous studies showed that weight-gaining rats had greater retention and reduced turnover of 125I-labeled tumor necrosis factor (TNF)-alpha in the circulation compared with weight-losing animals. We therefore tested the hypothesis that protein-energy restriction with weight loss reduces the levels of soluble TNF-alpha receptor (sTNFR) and membrane TNFR (mTNFR) and the cellular expression of TNF-alpha mRNA. Twenty-six male rats weighing 200-220 g were fed a liquid formula diet for 10 days and divided equally into weight-gaining rats meeting all nutritional requirements (WG rats) and weight-losing rats with protein-energy restriction (WL rats). 125I-TNF-alpha binding was demonstrated in plasma and plasma membrane to proteins of molecular masses of 92 and 243 kDa, a finding identical to that seen with purified human p55. Excess unlabeled TNF-alpha displaced the binding showing its specificity. The degree of binding to plasma protein and liver plasma membrane was markedly reduced in WL rats. Northern analysis showed that the expression of p55 mRNA was increased in the lungs and reduced in kidneys of WL compared with WG rats. The expression of p75 mRNA was not influenced by the nutritional status. We conclude that levels of sTNFR and mTNFR were reduced in WL rats. Reduced sTNFR and liver mTNFR are not due to a reduction in the expression of either p55 or p75 mRNA in WL rats. Reduced mTNFR, together with reduced shedding of soluble receptors, may have a protective role in WL rats.

electrophoresis; Northern blot; probes; plasma membranes


This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
N. Raina and K. N. Jeejeebhoy
Effect of low-protein diet and protein supplementation on the expressions of TNF-{alpha}, TNFR-I, and TNFR-II in organs and muscle of LPS-injected rats
Am J Physiol Endocrinol Metab, March 1, 2004; 286(3): E481 - E487.
[Abstract] [Full Text]

Home page
 Am. J. Clin. Nutr.Home page
K. N Jeejeebhoy
Total parenteral nutrition: potion or poison?
Am. J. Clinical Nutrition, August 1, 2001; 74(2): 160 - 163.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online