AJP - Endo Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 277: E380-E389, 1999;
0193-1849/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jekabsons, M. B.
Right arrow Articles by Horwitz, B. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jekabsons, M. B.
Right arrow Articles by Horwitz, B. A.
Vol. 277, Issue 2, E380-E389, August 1999

T3 stimulates resting metabolism and UCP-2 and UCP-3 mRNA but not nonphosphorylating mitochondrial respiration in mice

Mika B. Jekabsons1, Francine M. Gregoire2,3, Nancy A. Schonfeld-Warden2, Craig H. Warden2,3, and B. A. Horwitz1

1 Section of Neurobiology, Physiology, and Behavior, Division of Biological Sciences, and Departments of 2 Pediatrics and 3 Rowe Genetics, School of Medicine, University of California, Davis, California 95616

The molecular basis for variations in resting metabolic rate (RMR) within a species is unknown. One possibility is that variations in RMR occur because of variations in uncoupling protein 2 (UCP-2) and uncoupling protein 3 (UCP-3) expression, resulting in mitochondrial proton leak differences. We tested the hypothesis that UCP-2 and -3 mRNAs positively correlate with RMR and proton leak. We treated thyroidectomized and sham-operated mice with triiodothyronine (T3) or vehicle and measured RMR, liver, and skeletal muscle mitochondrial nonphosphorylating respiration and UCP-2 and -3 mRNAs. T3 stimulated RMR and liver UCP-2 and gastrocnemius UCP-2 and -3 expression. Mitochondrial respiration was not affected by T3 and did not correlate with UCP-2 and -3 mRNAs. Gastrocnemius UCP-2 and -3 expression did correlate with RMR. We conclude 1) T3 did not influence intrinsic mitochondrial properties such as membrane structure and composition, and 2) variations in UCP-2 and -3 expression may partly explain variations in RMR. One possible explanation for these data is that T3 stimulates the leak in vivo but not in vitro because a posttranslational regulator of UCP-2 and -3 is not retained in the mitochondrial fraction.

proton leak; thyroid hormone; mitochondria; uncoupling protein


This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
G. Medina-Gomez, R. M. Calvo, and M.-J. Obregon
Thermogenic effect of triiodothyroacetic acid at low doses in rat adipose tissue without adverse side effects in the thyroid axis
Am J Physiol Endocrinol Metab, April 1, 2008; 294(4): E688 - E697.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
B. D. Fink, J. A. Herlein, K. Almind, S. Cinti, C. R. Kahn, and W. I. Sivitz
Mitochondrial proton leak in obesity-resistant and obesity-prone mice
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2007; 293(5): R1773 - R1780.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. Mostyn, J. C. Litten, K. S. Perkins, P. J. Euden, A. M. Corson, M. E. Symonds, and L. Clarke
Influence of size at birth on the endocrine profiles and expression of uncoupling proteins in subcutaneous adipose tissue, lung, and muscle of neonatal pigs
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2005; 288(6): R1536 - R1542.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. C. Carreras, J. G. Peralta, D. P. Converso, P. V. Finocchietto, I. Rebagliati, A. A. Zaninovich, and J. J. Poderoso
Modulation of liver mitochondrial NOS is implicated in thyroid-dependent regulation of O2 uptake
Am J Physiol Heart Circ Physiol, December 1, 2001; 281(6): H2282 - H2288.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
P. M. Yen
Physiological and Molecular Basis of Thyroid Hormone Action
Physiol Rev, July 1, 2001; 81(3): 1097 - 1142.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
K. R. Short, J. Nygren, R. Barazzoni, J. Levine, and K. S. Nair
T3 increases mitochondrial ATP production in oxidative muscle despite increased expression of UCP2 and -3
Am J Physiol Endocrinol Metab, May 1, 2001; 280(5): E761 - E769.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
X. X. Yu, J. L. Barger, B. B. Boyer, M. D. Brand, G. Pan, and S. H. Adams
Impact of endotoxin on UCP homolog mRNA abundance, thermoregulation, and mitochondrial proton leak kinetics
Am J Physiol Endocrinol Metab, August 1, 2000; 279(2): E433 - E446.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
A. L. Hildebrandt and P. D. Neufer
Exercise attenuates the fasting-induced transcriptional activation of metabolic genes in skeletal muscle
Am J Physiol Endocrinol Metab, June 1, 2000; 278(6): E1078 - E1086.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
S. H. Adams
Uncoupling Protein Homologs: Emerging Views of Physiological Function
J. Nutr., April 1, 2000; 130(4): 711 - 714.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
J. A. Stuart, J. A. Harper, K. M. Brindle, M. B. Jekabsons, and M. D. Brand
Physiological Levels of Mammalian Uncoupling Protein 2 Do Not Uncouple Yeast Mitochondria
J. Biol. Chem., May 18, 2001; 276(21): 18633 - 18639.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Cadenas, K. S. Echtay, J. A. Harper, M. B. Jekabsons, J. A. Buckingham, E. Grau, A. Abuin, H. Chapman, J. C. Clapham, and M. D. Brand
The Basal Proton Conductance of Skeletal Muscle Mitochondria from Transgenic Mice Overexpressing or Lacking Uncoupling Protein-3
J. Biol. Chem., January 18, 2002; 277(4): 2773 - 2778.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online