AJP - Endo Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 277: E268-E276, 1999;
0193-1849/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lang, C. H.
Right arrow Articles by Vary, T. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lang, C. H.
Right arrow Articles by Vary, T. C.
Vol. 277, Issue 2, E268-E276, August 1999

Inhibition of muscle protein synthesis by alcohol is associated with modulation of eIF2B and eIF4E

Charles H. Lang, Duanqing Wu, Robert A. Frost, Leonard S. Jefferson, Scot R. Kimball, and Thomas C. Vary

Departments of Cellular and Molecular Physiology and Surgery, Pennsylvania State College of Medicine, Hershey, Pennsylvania 17033

The present study examined potential mechanisms for the inhibition of protein synthesis in skeletal muscle after chronic alcohol consumption. Rats were maintained on an alcohol-containing diet for 14 wk; control animals were pair fed. Alcohol-induced myopathy was confirmed by a reduction in lean body mass as well as a decrease in the weight of the gastrocnemius and psoas muscles normalized for tibial length. No alcohol-induced decrease in total RNA content (an estimate of ribosomal RNA) was detected in any muscle examined, suggesting that alcohol reduced translational efficiency but not the capacity for protein synthesis. To identify mechanisms responsible for regulating translational efficiency, we analyzed several eukaryotic initiation factors (eIF). There was no difference in the muscle content of either total eIF2alpha or the amount of eIF2alpha in the phosphorylated form between alcohol-fed and control rats. Similarly, the relative amount of eIF2Bepsilon in muscle was also not different. In contrast, alcohol decreased eIF2B activity in psoas (fast-twitch) but not in soleus or heart (slow-twitch) muscles. Alcohol feeding also dramatically influenced the distribution of eIF4E in the gastrocnemius (fast-twitch) muscle. Compared with control values, muscle from alcohol-fed rats demonstrated 1) an increased binding of the translational repressor 4E-binding protein 1 (4E-BP1) with eIF4E, 2) a decrease in the phosphorylated gamma -form of 4E-BP1, and 3) a decrease in eIF4G associated with eIF4E. In summary, these data suggest that chronic alcohol consumption impairs translation initiation in muscle by altering multiple regulatory sites, including eIF2B activity and eIF4E availability.

ethanol; peptide-chain initiation; translation initiation; eukaryotic initiation factors 2, 4E, and 4G; 4E-binding protein 1; adenosine 5'-triphosphate; rats


This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. J. Rose, B. Bisiani, B. Vistisen, B. Kiens, and E. A. Richter
Skeletal muscle eEF2 and 4EBP1 phosphorylation during endurance exercise is dependent on intensity and muscle fiber type
Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2009; 296(2): R326 - R333.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
A. M. Pruznak, L. Hong-Brown, R. Lantry, P. She, R. A. Frost, T. C. Vary, and C. H. Lang
Skeletal and cardiac myopathy in HIV-1 transgenic rats
Am J Physiol Endocrinol Metab, October 1, 2008; 295(4): E964 - E973.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
A. M. Karinch, J. H. Martin, and T. C. Vary
Acute and chronic ethanol consumption differentially impact pathways limiting hepatic protein synthesis
Am J Physiol Endocrinol Metab, July 1, 2008; 295(1): E3 - E9.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
T. C. Vary, R. A. Frost, and C. H. Lang
Acute alcohol intoxication increases atrogin-1 and MuRF1 mRNA without increasing proteolysis in skeletal muscle
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2008; 294(6): R1777 - R1789.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
C. H. Lang, R. A. Frost, and T. C. Vary
Skeletal muscle protein synthesis and degradation exhibit sexual dimorphism after chronic alcohol consumption but not acute intoxication
Am J Physiol Endocrinol Metab, June 1, 2007; 292(6): E1497 - E1506.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
T. C. Vary, S. R. Kimball, and A. Sumner
Sex-dependent differences in the regulation of myocardial protein synthesis following long-term ethanol consumption
Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2007; 292(2): R778 - R787.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
T. C. Vary, G. Deiter, and S. A. Goodman
Acute alcohol intoxication enhances myocardial eIF4G phosphorylation despite reducing mTOR signaling
Am J Physiol Heart Circ Physiol, January 1, 2005; 288(1): H121 - H128.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
C. H. Lang, R. A. Frost, E. Svanberg, and T. C. Vary
IGF-I/IGFBP-3 ameliorates alterations in protein synthesis, eIF4E availability, and myostatin in alcohol-fed rats
Am J Physiol Endocrinol Metab, June 1, 2004; 286(6): E916 - E926.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
A. A. Sneddon, M. Koll, M. C. Wallace, J. Jones, J. P. Miell, P. J. Garlick, and V. R. Preedy
Acute alcohol administration inhibits the refeeding response after starvation in rat skeletal muscle
Am J Physiol Endocrinol Metab, May 1, 2003; 284(5): E874 - E882.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
V. Kumar, R. A. Frost, and C. H. Lang
Alcohol impairs insulin and IGF-I stimulation of S6K1 but not 4E-BP1 in skeletal muscle
Am J Physiol Endocrinol Metab, November 1, 2002; 283(5): E917 - E928.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
P. Tessari, D. Bruttomesso, A. Pianta, M. Vettore, M. Zanetti, E. Kiwanuka, and E. Iori
Effects of wine intake on postprandial plasma amino acid and protein kinetics in type 1 diabetes
Am. J. Clinical Nutrition, May 1, 2002; 75(5): 856 - 866.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
T. C. Vary, C. J. Lynch, and C. H. Lang
Effects of chronic alcohol consumption on regulation of myocardial protein synthesis
Am J Physiol Heart Circ Physiol, September 1, 2001; 281(3): H1242 - H1251.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
C. H. Lang, R. A. Frost, V. Kumar, and T. C. Vary
Impaired myocardial protein synthesis induced by acute alcohol intoxication is associated with changes in eIF4F
Am J Physiol Endocrinol Metab, November 1, 2000; 279(5): E1029 - E1038.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
C. H. Lang, R. A. Frost, A. C. Nairn, D. A. MacLean, and T. C. Vary
TNF-alpha impairs heart and skeletal muscle protein synthesis by altering translation initiation
Am J Physiol Endocrinol Metab, February 1, 2002; 282(2): E336 - E347.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online