| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Copenhagen Muscle Research Center, Rigshospitalet, and Department of Medical Physiology, The Panum Institute, University of Copenhagen, 2200 Copenhagen N, Denmark
We questioned the
general view that contraction-induced muscle glucose transport only
depends on stimulation frequency and not on workload. Incubated soleus
muscles were electrically stimulated at a given pattern for 5 min.
Resting length was adjusted to achieve either no force (0% P), maximum
force (100% P), or 50% of maximum force (50% P). Glucose transport
(2-deoxy-D-glucose uptake)
increased directly with force development
(P < 0.05) [27 ± 2 (basal), 45 ± 2 (0% P), 68 ± 3 (50% P), and 94 ± 3 (100%
P)
nmol · g
1 · 5 min1]. Glycogen
decreased at 0% P but did not change further with force development
(P > 0.05). Lactate, AMP, and IMP
concentrations were higher (P < 0.05) and ATP concentrations lower
(P < 0.05) when force was
produced than when it was not. 5'-AMP-activated protein kinase
(AMPK) activity increased directly with force [20 ± 2 (basal), 60 ± 11 (0% P), 91 ± 12 (50% P), and 109 ± 12 (100% P)
pmol · mg1 · min1].
Passive stretch (~86% P) doubled glucose transport without altering
metabolism. In conclusion, contraction-induced muscle glucose transport
varies directly with force development and is not solely determined by
stimulation frequency. AMPK activity is probably an essential
determinant of contraction-induced glucose transport. In contrast,
glycogen concentrations per se do not play a major role. Finally,
passive stretch per se increases glucose transport in muscle.
2-deoxy-D-glucose; metabolism; exercise; GLUT-4; signal transduction
This article has been cited by other articles:
|
|
 |
|
  M. E. Sandstrom, S.-J. Zhang, H. Westerblad, and A. Katz Mechanical load plays little role in contraction-mediated glucose transport in mouse skeletal muscle J. Physiol., March 1, 2007; 579(2): 527 - 534. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Fujii, N. Jessen, and L. J. Goodyear AMP-activated protein kinase and the regulation of glucose transport Am J Physiol Endocrinol Metab, November 1, 2006; 291(5): E867 - E877. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. A Walz, L. Harndahl, N. Wierup, E. Zmuda-Trzebiatowska, F. Svennelid, V. C Manganiello, T. Ploug, F. Sundler, E. Degerman, B. Ahren, et al. Early and rapid development of insulin resistance, islet dysfunction and glucose intolerance after high-fat feeding in mice overexpressing phosphodiesterase 3B. J. Endocrinol., June 1, 2006; 189(3): 629 - 641. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yamaguchi, H. Katahira, S. Ozawa, Y. Nakamichi, T. Tanaka, T. Shimoyama, K. Takahashi, K. Yoshimoto, M. O. Imaizumi, S. Nagamatsu, et al. Activators of AMP-activated protein kinase enhance GLUT4 translocation and its glucose transport activity in 3T3-L1 adipocytes Am J Physiol Endocrinol Metab, October 1, 2005; 289(4): E643 - E649. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Rose and E. A. Richter Skeletal Muscle Glucose Uptake During Exercise: How is it Regulated? Physiology, August 1, 2005; 20(4): 260 - 270. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Jessen and L. J. Goodyear Contraction signaling to glucose transport in skeletal muscle J Appl Physiol, July 1, 2005; 99(1): 330 - 337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Sancho, J. Kim, and G. D. Cartee Decreased contraction-stimulated glucose transport in isolated epitrochlearis muscles of pregnant rats J Appl Physiol, March 1, 2005; 98(3): 1021 - 1027. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Morita, K. Iizuka, K. Okita, T. Oikawa, K. Yonezawa, T. Nagai, Y. Tokumitsu, T. Murakami, A. Kitabatake, and H. Kawaguchi Exposure to pressure stimulus enhances succinate dehydrogenase activity in L6 myoblasts Am J Physiol Endocrinol Metab, December 1, 2004; 287(6): E1064 - E1069. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. J. Durham, S. L. Miller, C. W. Yeckel, D. L. Chinkes, K. D. Tipton, B. B. Rasmussen, and R. R. Wolfe Leg glucose and protein metabolism during an acute bout of resistance exercise in humans J Appl Physiol, October 1, 2004; 97(4): 1379 - 1386. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. KJAeR Role of Extracellular Matrix in Adaptation of Tendon and Skeletal Muscle to Mechanical Loading Physiol Rev, April 1, 2004; 84(2): 649 - 698. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Sakamoto, W. G. Aschenbach, M. F. Hirshman, and L. J. Goodyear Akt signaling in skeletal muscle: regulation by exercise and passive stretch Am J Physiol Endocrinol Metab, November 1, 2003; 285(5): E1081 - E1088. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ai, E. Ralston, H. P. M. M. Lauritzen, H. Galbo, and T. Ploug Disruption of microtubules in rat skeletal muscle does not inhibit insulin- or contraction-stimulated glucose transport Am J Physiol Endocrinol Metab, October 1, 2003; 285(4): E836 - E844. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Mulder, M. Sorhede-Winzell, J. A. Contreras, M. Fex, K. Strom, T. Ploug, H. Galbo, P. Arner, C. Lundberg, F. Sundler, et al. Hormone-sensitive Lipase Null Mice Exhibit Signs of Impaired Insulin Sensitivity whereas Insulin Secretion Is Intact J. Biol. Chem., September 19, 2003; 278(38): 36380 - 36388. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Sakamoto and L. J. Goodyear Exercise Effects on Muscle Insulin Signaling and Action: Invited Review: Intracellular signaling in contracting skeletal muscle J Appl Physiol, July 1, 2002; 93(1): 369 - 383. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ai, J. Ihlemann, Y. Hellsten, H. P. M. M. Lauritzen, D. G. Hardie, H. Galbo, and T. Ploug Effect of fiber type and nutritional state on AICAR- and contraction-stimulated glucose transport in rat muscle Am J Physiol Endocrinol Metab, June 1, 2002; 282(6): E1291 - E1300. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Bergeron, J. M. Ren, K. S. Cadman, I. K. Moore, P. Perret, M. Pypaert, L. H. Young, C. F. Semenkovich, and G. I. Shulman Chronic activation of AMP kinase results in NRF-1 activation and mitochondrial biogenesis Am J Physiol Endocrinol Metab, December 1, 2001; 281(6): E1340 - E1346. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Aslesen, E. M. L. Engebretsen, J. Franch, and J. Jensen Glucose uptake and metabolic stress in rat muscles stimulated electrically with different protocols J Appl Physiol, September 1, 2001; 91(3): 1237 - 1244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. C. Martineau and P. F. Gardiner Insight into skeletal muscle mechanotransduction: MAPK activation is quantitatively related to tension J Appl Physiol, August 1, 2001; 91(2): 693 - 702. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Musi, N. Fujii, M. F. Hirshman, I. Ekberg, S. Fröberg, O. Ljungqvist, A. Thorell, and L. J. Goodyear AMP-Activated Protein Kinase (AMPK) Is Activated in Muscle of Subjects With Type 2 Diabetes During Exercise Diabetes, May 1, 2001; 50(5): 921 - 927. [Abstract] [Full Text]
|
|
|
|
|
|
J. Ihlemann, T. Ploug, Y. Hellsten, and H. Galbo Effect of stimulation frequency on contraction-induced glucose transport in rat skeletal muscle Am J Physiol Endocrinol Metab, October 1, 2000; 279(4): E862 - E867. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kristiansen, J. Gade, J. F. P. Wojtaszewski, B. Kiens, and E. A. Richter Glucose uptake is increased in trained vs. untrained muscle during heavy exercise J Appl Physiol, September 1, 2000; 89(3): 1151 - 1158. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
