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1 Departments of Surgery,
2 Neurology, and
3 Molecular and Cellular
Physiology,
Epinephrine and amylin stimulate glycogenolysis,
glycolysis, and
Na+-K+-ATPase
activity in skeletal muscle. However, it is not known whether these
hormones stimulate glycolytic ATP production that is specifically
coupled to ATP consumption by the
Na+-K+
pump. These studies correlated glycolysis with
Na+-K+-ATPase
activity in resting rat extensor digitorum longus and soleus muscles
incubated at 30°C in well-oxygenated medium. Lactate production
rose three- to fourfold, and the intracellular
Na+-to-K+
ratio
(Na+/K+)
fell with increasing concentrations of epinephrine or amylin. In
muscles exposed to epinephrine at high concentrations (5 × 10
7 and 5 × 106 M), ouabain
significantly inhibited glycolysis by ~70% in either muscle and
inhibited glycogenolysis by ~40 and ~75% in extensor digitorum
longus and soleus, respectively. In the absence of ouabain, but not in
its presence, statistically significant inverse correlations were
observed between lactate production and intracellular
Na+/K+
for each hormone. Epinephrine had no significant effect on oxygen consumption or ATP content in either muscle. These results suggest for
the first time that stimulation of glycolysis and glycogenolysis in
resting skeletal muscle by epinephrine or amylin is closely linked to
stimulation of active
Na+-K+ transport.
ouabain; lactate; oxygen consumption; metabolic compartmentation
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