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1 Copenhagen Muscle Research Centre, August Krogh Institute, University of Copenhagen, DK-2100 Copenhagen, Denmark; and 2 Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1 TD, United Kingdom
The profiles of the
lactate/H+ transporter isoforms
[monocarboxylate transporter isoforms (MCT)] MCT1 and MCT4
(formerly MCT3 of Price, N. T., V. N. Jackson, and A. P. Halestrap.
Biochem. J. 329: 321-328, 1998)
were studied in the soleus, triceps brachii, and vastus lateralis
muscles of six male subjects. The fiber-type compositions of the
muscles were evaluated from the occurrence of the myosin heavy chain
isoforms, and the fibers were classified as type I, IIA, or IIX. The
total content of MCT1 and MCT4 was determined in muscle homogenates by
Western blotting, and MCT1 and MCT4 were visualized on cross-sectional
muscle sections by immunofluorescence microscopy. The Western blotting
revealed a positive, linear relationship between the MCT1 content and
the occurrence of type I fibers in the muscle, but no significant relation was found between MCT4 content and fiber type. Moreover, the
interindividual variation in MCT4 content was much larger than the
interindividual variation in MCT1 content in homogenate samples. The
immunofluorescence microscopy showed that within a given muscle
section, the MCT4 isoform was clearly more abundant in type II fibers
than in type I fibers, whereas only minor differences existed in the
occurrence of the MCT1 isoform between type I and II fibers. Together
the present results indicate that the content of MCT1 in a muscle
varies between different muscles, whereas fiber-type differences in
MCT1 content are minor within a given muscle section. In contrast, the
content of MCT4 is clearly fiber-type specific but apparently quite
similar in various muscles.
immunofluorescence microscopy; Western blotting; monocarboxylate transporter; fiber type
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