| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Physiology, Midwestern University, Glendale, Arizona 85308
Vascular smooth muscle cells (VSMC) subjected to
acute or chronic stretch display enhanced growth rates in vitro and in
vivo. Clinical examples of vascular hyperplasia (e.g., systolic
hypertension and postinjury restenosis) suggest that local insulin-like
growth factor I (IGF-I) expression is enhanced. Therefore, we
investigated the role of in vitro cyclic stretch on rat VSMC IGF-I
secretion and cellular growth. In serum-free medium, cyclic stretch (1 Hz at 120% resting length for 48 h) stimulated thymidine incorporation ~40% above that seen in nonstretched cells. Graded stretch magnitude (100-125% resting length) yielded graded increases in VSMC
growth. Exogenous IGF-I increased growth of serum-starved, nonstretched VSMC in a dose-dependent manner, with maximal growth seen with 10
7 M. IGF-I secretion from
stretched cells was 20- to 30-fold greater than from those cells
cultured in a static environment. Stretch-induced increases in growth
were completely blocked on addition of anti-IGF-I and partially blocked
with platelet-derived growth factor (PDGF) antibodies and with a
tyrosine kinase inhibitor (tyrphostin-1). Finally, blockade of
stretch-activated cation channels with
GdCl3 profoundly inhibited
stretch-induced growth. We conclude that stretch increases VSMC IGF-I
secretion and that such autocrine IGF-I is required for stretch-induced
growth. PDGF and stretch-sensitive cation channels are likely
additional components of a complex pathway that regulates
stretch-induced VSMC seen in systolic hypertension and postinjury restenosis.
insulin-like growth factor I; restenosis; platelet-derived growth factor; tyrosine kinase
This article has been cited by other articles:
|
|
 |
|
  J. P. Stegemann, H. Hong, and R. M. Nerem Mechanical, biochemical, and extracellular matrix effects on vascular smooth muscle cell phenotype J Appl Physiol, June 1, 2005; 98(6): 2321 - 2327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Goldman, L. Zhong, and S. Q. Liu Degradation of alpha -actin filaments in venous smooth muscle cells in response to mechanical stretch Am J Physiol Heart Circ Physiol, May 1, 2003; 284(5): H1839 - H1847. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hayashi, K. Mori, and H. Miyazaki Biomechanical response of femoral vein to chronic elevation of blood pressure in rabbits Am J Physiol Heart Circ Physiol, February 1, 2003; 284(2): H511 - H518. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Galvin, R. W. G. Watson, J. I. Gillespie, H. Brady, and J. M. Fitzpatrick Mechanical stretch regulates cell survival in human bladder smooth muscle cells in vitro Am J Physiol Renal Physiol, December 1, 2002; 283(6): F1192 - F1199. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. R. Standley, M. A. Stanley, and P. Senechal Activation of mitogenic and antimitogenic pathways in cyclically stretched arterial smooth muscle Am J Physiol Endocrinol Metab, December 1, 2001; 281(6): E1165 - E1171. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. C. Berk Vascular Smooth Muscle Growth: Autocrine Growth Mechanisms Physiol Rev, July 1, 2001; 81(3): 999 - 1030. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Loennechen, A. Stoylen, V. Beisvag, U. Wisloff, and O. Ellingsen Regional expression of endothelin-1, ANP, IGF-1, and LV wall stress in the infarcted rat heart Am J Physiol Heart Circ Physiol, June 1, 2001; 280(6): H2902 - H2910. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
