| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Physiology and Lipid Research Unit, Laval University Hospital Research Center, Quebec, Canada G1V 4G2
The aim of the present study was to investigate
the mechanism of adipose tissue inducible nitric oxide synthase (iNOS)
induction in endotoxemia. Systemic administration of the bacterial
endotoxin lipopolysaccharide (LPS) to rats for 
8 h markedly increased
iNOS mRNA and protein levels in white and brown adipose tissues. This effect was comparable to or greater than the induction of iNOS in
liver, kidney, or skeletal muscle. iNOS activity was also found to be
greatly enhanced in both white and brown adipose tissues of LPS-treated
rats (an ~12- to 20-fold increase). Treatment of cultured 3T3-L1
adipocytes with LPS, tumor necrosis factor-
(TNF-), or
interferon-
(IFN-) alone failed to induce iNOS activity. However,
when used in combination, TNF-, IFN-, and LPS markedly and
synergistically increased iNOS activity in these cells. In conclusion,
these results suggest that adipose tissue is a major site of iNOS
expression in endotoxemia. Our data further indicate that iNOS
induction can be reproduced in vitro in cultured adipocytes and that a
concerted action of cytokines and endotoxin is needed for maximal
activation of the enzyme.
lipopolysaccharide; tumor necrosis factor-; interferon-; adipocytes; skeletal muscle; liver; kidney
This article has been cited by other articles:
|
|
 |
|
  J. R. Pauli, E. R. Ropelle, D. E. Cintra, M. A. Carvalho-Filho, J. C. Moraes, C. T. De Souza, L. A. Velloso, J. B. C. Carvalheira, and M. J. A. Saad Acute physical exercise reverses S-nitrosation of the insulin receptor, insulin receptor substrate 1 and protein kinase B/Akt in diet-induced obese Wistar rats J. Physiol., January 15, 2008; 586(2): 659 - 671. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Linscheid, D. Seboek, H. Zulewski, A. Scherberich, N. Blau, U. Keller, and B. Muller Cytokine-induced metabolic effects in human adipocytes are independent of endogenous nitric oxide Am J Physiol Endocrinol Metab, June 1, 2006; 290(6): E1068 - E1077. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. H. Berg, Y. Lin, M. P. Lisanti, and P. E. Scherer Adipocyte differentiation induces dynamic changes in NF-{kappa}B expression and activity Am J Physiol Endocrinol Metab, December 1, 2004; 287(6): E1178 - E1188. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Merial, A. Bouloumie, V. Trocheris, M. Lafontan, and J. Galitzky Nitric oxide-dependent downregulation of adipocyte UCP-2 expression by tumor necrosis factor-alpha Am J Physiol Cell Physiol, October 1, 2000; 279(4): C1100 - C1106. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Lin, H. Lee, A. H. Berg, M. P. Lisanti, L. Shapiro, and P. E. Scherer The Lipopolysaccharide-activated Toll-like Receptor (TLR)-4 Induces Synthesis of the Closely Related Receptor TLR-2 in Adipocytes J. Biol. Chem., August 4, 2000; 275(32): 24255 - 24263. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
