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Am J Physiol Endocrinol Metab 276: E401-E408, 1999;
0193-1849/99 $5.00
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Vol. 276, Issue 2, E401-E408, February 1999

SPECIAL COMMUNICATION
Direct access to interstitial fluid in adipose tissue in humans by use of open-flow microperfusion

L. Schaupp1,2, M. Ellmerer1,2, G. A. Brunner2, A. Wutte2, G. Sendlhofer2, Z. Trajanoski1, F. Skrabal3, T. R. Pieber2, and P. Wach1

1 Department of Biophysics, Institute of Biomedical Engineering, Graz University of Technology, A-8010 Graz; 2 Department of Internal Medicine, Diabetes and Metabolism, Karl Franzens University Graz, A-8036 Graz; and 3 Department of Internal Medicine, Krankenhaus der Barmherzigen Brüder, Teaching Hospital, Karl Franzens University Graz, A-8020 Graz, Austria

To gain direct access to the interstitial fluid (ISF), a new technique called open-flow microperfusion has been evaluated. This method is based on a double-lumen catheter with macroscopic (0.3-0.5 mm diameter) perforations that is inserted into the subcutaneous adipose tissue and constantly perfused. Thus partial equilibration between the ISF and the perfusion fluid occurs. The glucose concentration of the ISF was determined by established (zero flow rate, no net flux, and recirculation procedures) and new (ionic reference and suction technique) calibration methods by use of open-flow microperfusion. The data show that 1) the glucose concentration in the ISF is significantly lower than the corresponding arterialized venous plasma values during basal steady-state conditions (adipose tissue 3.2 ± 0.10 mM, plasma 5.27 ± 0.12 mM) as well as during hyperglycemic clamp experiments (adipose tissue 7.3 ± 0.13 mM, plasma 9.91 ± 0.16 mM), and 2) it is possible to determine the recovery continuously by using the ion concentration of the ISF as an internal standard (ionic reference).

glucose; ionic reference; mass transfer resistance; hyperglycemia


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