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1 Copenhagen Muscle Research Centre, The August Krogh Institute, University of Copenhagen, 2100 Copenhagen, Denmark; and 2 Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1 TD, United Kingdom
The present study examined the effect of
high-intensity exercise training on muscle sarcolemmal
lactate/H+ transport
and the monocarboxylate transporters (MCT1 and MCT4) as well as lactate
and H+ release during intense
exercise in humans. One-legged knee-extensor exercise training was
performed for 8 wk, and biopsies were obtained from untrained and
trained vastus lateralis muscle. The rate of lactate/H+ transport determined in
sarcolemmal giant vesicles was 12% higher (P < 0.05) in the trained than in
untrained muscle (n = 7). The content
of MCT1 and MCT4 protein was also higher (76 and 32%, respectively;
n = 4) in trained muscle. Release of
lactate and H+ from the quadriceps
muscle at the end of intense exhaustive knee-extensor exercise was
similar in the trained and untrained leg, although the estimated muscle
intracellular-to-interstitial gradients of lactate and
H+ were lower
(P < 0.05) in the trained than in
the untrained muscle. The present data show that intense exercise
training can increase lactate/H+
transport capacity in human skeletal muscle as well as improve the
ability of the muscle to release lactate and
H+ during contractions.
monocarboxylate transporters; blood flow; pH
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