AJP - Endo Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 275: E717-E728, 1998;
0193-1849/98 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Livesey, G.
Right arrow Articles by Greenwood, R. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Livesey, G.
Right arrow Articles by Greenwood, R. H.
Vol. 275, Issue 4, E717-E728, October 1998

MODELING IN PHYSIOLOGY
Simultaneous time-varying systemic appearance of oral and hepatic glucose in adults monitored with stable isotopes

G. Livesey1, P. D. G. Wilson2, J. R. Dainty2, J. C. Brown1, R. M. Faulks1, M. A. Roe1, T. A. Newman1, J. Eagles1, F. A. Mellon1, and R. H. Greenwood3

Departments of 1 Nutrition, Diet, and Health and of 2 Food Biophysics, Institute of Food Research, Norwich NR4 7UA; and 3 Department of Medicine, Norfolk and Norwich Hospital, Norwich NR1 3SR, United Kingdom

The rates (and extent) of appearance of glucose in arterialized plasma from an oral glucose load and from liver (RaO, RaH) can be estimated in humans using radioisotopes, but estimates vary among laboratories. We investigated the use of stable isotopes and undertook 22 primed intravenous infusions of D-[6,6-2H2]glucose with an oral load including D-[13C6]glucose in healthy humans. The effective glucose pool volume (VS) had a lower limit of 230 ml/kg body weight (cf. 130 ml/kg commonly assumed). This VS in Steele's one-compartment model of glucose kinetics gave a systemic appearance from a 50-g oral glucose load per 70 kg body weight of 96 ± 3% of that ingested, which compared with a theoretical value of ~95%. Mari's two-compartment model gave 100 ± 3%. The two models gave practically identical RaO and RaH at each point in time and a plateau in the cumulative RaO when absorption was complete. Less than 3% of 13C was recycled to [13C3]glucose, suggesting that recycling errors were practically negligible in this study. Causes of variation among laboratories are identified. We conclude that stable isotopes provide a reliable and safe alternative to radioactive isotopes in these studies.

metabolism; absorption; hepatic production; modeling


This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
G. Toffolo, R. Basu, C. Dalla Man, R. Rizza, and C. Cobelli
Assessment of postprandial glucose metabolism: conventional dual- vs. triple-tracer method
Am J Physiol Endocrinol Metab, October 1, 2006; 291(4): E800 - E806.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
F. Fery, L. Tappy, J. Deviere, and E. O. Balasse
Comparison of intraduodenal and intravenous glucose metabolism under clamp conditions in humans
Am J Physiol Endocrinol Metab, February 1, 2004; 286(2): E176 - E183.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
R. Basu, B. Di Camillo, G. Toffolo, A. Basu, P. Shah, A. Vella, R. Rizza, and C. Cobelli
Use of a novel triple-tracer approach to assess postprandial glucose metabolism
Am J Physiol Endocrinol Metab, January 1, 2003; 284(1): E55 - E69.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
L. Noah, M. Krempf, G. Lecannu, P. Maugere, and M. Champ
Bioavailability of starch and postprandial changes in splanchnic glucose metabolism in pigs
Am J Physiol Endocrinol Metab, February 1, 2000; 278(2): E181 - E188.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
G. Livesey, P. D. G. Wilson, M. A. Roe, R. M. Faulks, L. M. Oram, J. C. Brown, J. Eagles, R. H. Greenwood, and H. Kennedy
Splanchnic retention of intraduodenal and intrajejunal glucose in healthy adults
Am J Physiol Endocrinol Metab, October 1, 1998; 275(4): E709 - E716.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online