|
|
||||||||
1 Department of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1; 2 The Copenhagen Muscle Research Centre, August Krogh Institute, University of Copenhagen, DK-2100 Copenhagen, Denmark; 3 Department of Exercise Sciences, University of Southern California, Los Angeles, California 90089; and 4 Department of Physiology, Cardiovascular Research Institute Maastricht, Maastricht University, 6200 MD Maastricht, The Netherlands
We performed studies
1) to investigate the kinetics of
palmitate transport into giant sarcolemmal vesicles,
2) to determine whether the
transport capacity is greater in red muscles than in white muscles, and
3) to determine whether putative
long-chain fatty acid (LCFA) transporters are more abundant in red than
in white muscles. For these studies we used giant sarcolemmal vesicles, which contained cytoplasmic fatty acid binding protein
(FABPc), an intravesicular fatty
acid sink. Intravesicular FABPc
concentrations were sufficiently high so as not to limit the uptake of
palmitate under conditions of maximal palmitate uptake (i.e., 4.5-fold
excess in white and 31.3-fold excess in red muscle vesicles). All of the palmitate taken up was recovered as unesterified palmitate. Palmitate uptake was reduced by phloretin (
50%),
sulfo-N-succinimidyl oleate
(
43%), anti-plasma membrane-bound FABP
(FABPpm,
30%), trypsin
(
45%), and when incubation temperature was lowered to 0°C
(
70%). Palmitate uptake was also reduced by excess oleate (
65%), but not by excess octanoate or by glucose. Kinetic
studies showed that maximal transport was 1.8-fold greater in red
vesicles than in white vesicles. The Michaelis-Menten constant in both types of vesicles was ~6 nM. Fatty acid transport protein mRNA and
fatty acid translocase (FAT) mRNA were about fivefold greater in red
muscles than in white muscles. FAT/CD36 and
FABPpm proteins in red vesicles or
in homogenates were greater than in white vesicles or homogenates
(P < 0.05). These studies provide
the first evidence of a protein-mediated LCFA transport system in
skeletal muscle. In this tissue, palmitate transport rates are greater
in red than in white muscles because more LCFA transporters are
available.
fatty acid translocase; fatty acid transport protein; plasma membrane-bound fatty acid binding protein; giant sarcolemmal vesicles
This article has been cited by other articles:
![]() |
C. R. Benton, G. P. Holloway, S. E. Campbell, Y. Yoshida, N. N. Tandon, J. F. C. Glatz, J. J. J. F. P. Luiken, L. L. Spriet, and A. Bonen Rosiglitazone increases fatty acid oxidation and fatty acid translocase (FAT/CD36) but not carnitine palmitoyltransferase I in rat muscle mitochondria J. Physiol., March 15, 2008; 586(6): 1755 - 1766. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Berthiaume, M. Laplante, W. T. Festuccia, K. Cianflone, L. P. Turcotte, D. R. Joanisse, G. Olivecrona, R. Thieringer, and Y. Deshaies 11beta-HSD1 inhibition improves triglyceridemia through reduced liver VLDL secretion and partitions lipids toward oxidative tissues Am J Physiol Endocrinol Metab, October 1, 2007; 293(4): E1045 - E1052. [Abstract] [Full Text] [PDF] |
||||
![]() |
H. Alkhateeb, A. Chabowski, J. F. C. Glatz, J. F. P. Luiken, and A. Bonen Two phases of palmitate-induced insulin resistance in skeletal muscle: impaired GLUT4 translocation is followed by a reduced GLUT4 intrinsic activity Am J Physiol Endocrinol Metab, September 1, 2007; 293(3): E783 - E793. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. J. Dube, B. A. Bhatt, N. Dedousis, A. Bonen, and R. M. O'Doherty Leptin, skeletal muscle lipids, and lipid-induced insulin resistance Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2007; 293(2): R642 - R650. [Abstract] [Full Text] [PDF] |
||||
![]() |
Y. Yoshida, G. P. Holloway, V. Ljubicic, H. Hatta, L. L. Spriet, D. A. Hood, and A. Bonen Negligible direct lactate oxidation in subsarcolemmal and intermyofibrillar mitochondria obtained from red and white rat skeletal muscle J. Physiol., August 1, 2007; 582(3): 1317 - 1335. [Abstract] [Full Text] [PDF] |
||||
![]() |
G. P. Holloway, J. Lally, J. G. Nickerson, H. Alkhateeb, L. A. Snook, G. J. F. Heigenhauser, J. Calles-Escandon, J. F. C. Glatz, J. J. F. P. Luiken, L. L. Spriet, et al. Fatty acid binding protein facilitates sarcolemmal fatty acid transport but not mitochondrial oxidation in rat and human skeletal muscle J. Physiol., July 1, 2007; 582(1): 393 - 405. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Bonen, X.-X. Han, D. D. J. Habets, M. Febbraio, J. F. C. Glatz, and J. J. F. P. Luiken A null mutation in skeletal muscle FAT/CD36 reveals its essential role in insulin- and AICAR-stimulated fatty acid metabolism Am J Physiol Endocrinol Metab, June 1, 2007; 292(6): E1740 - E1749. [Abstract] [Full Text] [PDF] |
||||
![]() |
G. P. Holloway, A. B. Thrush, G. J. F. Heigenhauser, N. N. Tandon, D. J. Dyck, A. Bonen, and L. L. Spriet Skeletal muscle mitochondrial FAT/CD36 content and palmitate oxidation are not decreased in obese women Am J Physiol Endocrinol Metab, June 1, 2007; 292(6): E1782 - E1789. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. C. Carpentier, F. Frisch, P. Brassard, F. Lavoie, A. Bourbonnais, D. Cyr, R. Giguere, and J.-P. Baillargeon Mechanism of insulin-stimulated clearance of plasma nonesterified fatty acids in humans Am J Physiol Endocrinol Metab, March 1, 2007; 292(3): E693 - E701. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Bonen, A. Chabowski, J. J. F. P Luiken, and J. F. C. Glatz Mechanisms and Regulation of Protein-Mediated Cellular Fatty Acid Uptake: Molecular, Biochemical, and Physiological Evidence Physiology, February 1, 2007; 22(1): 15 - 28. [Full Text] [PDF] |
||||
![]() |
T. Enoki, Y. Yoshida, J. Lally, H. Hatta, and A. Bonen Testosterone increases lactate transport, monocarboxylate transporter (MCT) 1 and MCT4 in rat skeletal muscle J. Physiol., November 15, 2006; 577(1): 433 - 443. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Chabowski, J. C. Chatham, N. N. Tandon, J. Calles-Escandon, J. F. C. Glatz, J. J. F. P. Luiken, and A. Bonen Fatty acid transport and FAT/CD36 are increased in red but not in white skeletal muscle of ZDF rats Am J Physiol Endocrinol Metab, September 1, 2006; 291(3): E675 - E682. [Abstract] [Full Text] [PDF] |
||||
![]() |
L. W. Castellani, P. Gargalovic, M. Febbraio, S. Charugundla, M.-L. Jien, and A. J. Lusis Mechanisms mediating insulin resistance in transgenic mice overexpressing mouse apolipoprotein A-II J. Lipid Res., December 1, 2004; 45(12): 2377 - 2387. [Abstract] [Full Text] [PDF] |
||||
![]() |
B. Kiens, C. Roepstorff, J. F. C. Glatz, A. Bonen, P. Schjerling, J. Knudsen, and J. N. Nielsen Lipid-binding proteins and lipoprotein lipase activity in human skeletal muscle: influence of physical activity and gender J Appl Physiol, October 1, 2004; 97(4): 1209 - 1218. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Chabowski, S. L. M. Coort, J. Calles-Escandon, N. N. Tandon, J. F. C. Glatz, J. J. F. P. Luiken, and A. Bonen Insulin stimulates fatty acid transport by regulating expression of FAT/CD36 but not FABPpm Am J Physiol Endocrinol Metab, October 1, 2004; 287(4): E781 - E789. [Abstract] [Full Text] [PDF] |
||||
![]() |
D. P. Y. Koonen, C. R. Benton, Y. Arumugam, N. N. Tandon, J. Calles-Escandon, J. F. C. Glatz, J. J. F. P. Luiken, and A. Bonen Different mechanisms can alter fatty acid transport when muscle contractile activity is chronically altered Am J Physiol Endocrinol Metab, June 1, 2004; 286(6): E1042 - E1049. [Abstract] [Full Text] [PDF] |
||||
![]() |
D. C. Clarke, D. Miskovic, X.-X. Han, J. Calles-Escandon, J. F. C. Glatz, J. J. F. P. Luiken, J. J. Heikkila, and A. Bonen Overexpression of membrane-associated fatty acid binding protein (FABPpm) in vivo increases fatty acid sarcolemmal transport and metabolism Physiol Genomics, March 12, 2004; 17(1): 31 - 37. [Abstract] [Full Text] [PDF] |
||||
![]() |
B. Mittendorfer, D. A. Fields, and S. Klein Excess body fat in men decreases plasma fatty acid availability and oxidation during endurance exercise Am J Physiol Endocrinol Metab, March 1, 2004; 286(3): E354 - E362. [Abstract] [Full Text] |
||||
![]() |
J. G. Richards, A. Bonen, G. J. F. Heigenhauser, and C. M. Wood Palmitate movement across red and white muscle membranes of rainbow trout Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2004; 286(1): R46 - R53. [Abstract] [Full Text] |
||||
![]() |
B. Binas, X.-X. Han, E. Erol, J. J. F. P. Luiken, J. F. C. Glatz, D. J. Dyck, R. Motazavi, P. J. Adihetty, D. A. Hood, and A. Bonen A null mutation in H-FABP only partially inhibits skeletal muscle fatty acid metabolism Am J Physiol Endocrinol Metab, September 1, 2003; 285(3): E481 - E489. [Abstract] [Full Text] [PDF] |
||||
![]() |
Y. Wang, M. Tonouchi, D. Miskovic, H. Hatta, and A. Bonen T3 increases lactate transport and the expression of MCT4, but not MCT1, in rat skeletal muscle Am J Physiol Endocrinol Metab, September 1, 2003; 285(3): E622 - E628. [Abstract] [Full Text] [PDF] |
||||
![]() |
H. M. Wilmsen, T. P. Ciaraldi, L. Carter, N. Reehman, S. R. Mudaliar, and R. R. Henry Thiazolidinediones upregulate impaired fatty acid uptake in skeletal muscle of type 2 diabetic subjects Am J Physiol Endocrinol Metab, August 1, 2003; 285(2): E354 - E362. [Abstract] [Full Text] [PDF] |
||||
![]() |
D. Cameron-Smith, L. M Burke, D. J Angus, R. J Tunstall, G. R Cox, A. Bonen, J. A Hawley, and M. Hargreaves A short-term, high-fat diet up-regulates lipid metabolism and gene expression in human skeletal muscle Am. J. Clinical Nutrition, February 1, 2003; 77(2): 313 - 318. [Abstract] [Full Text] [PDF] |
||||
![]() |
D. J. Calsbeek, T. L. Thompson, J. A. Dahl, N. R. Stob, J. T. Brozinick Jr., J. O. Hill, and M. S. Hickey Metabolic and anthropometric factors related to skeletal muscle UCP3 gene expression in healthy human adults Am J Physiol Endocrinol Metab, October 1, 2002; 283(4): E631 - E637. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. J. F. P. Luiken, Y. Arumugam, R. C. Bell, J. Calles-Escandon, N. N. Tandon, J. F. C. Glatz, and A. Bonen Changes in fatty acid transport and transporters are related to the severity of insulin deficiency Am J Physiol Endocrinol Metab, September 1, 2002; 283(3): E612 - E621. [Abstract] [Full Text] [PDF] |
||||
![]() |
B. Mittendorfer, J. F. Horowitz, and S. Klein Effect of gender on lipid kinetics during endurance exercise of moderate intensity in untrained subjects Am J Physiol Endocrinol Metab, July 1, 2002; 283(1): E58 - E65. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. G. Guglielmo, N. H. Haunerland, P. W. Hochachka, and T. D. Williams Seasonal dynamics of flight muscle fatty acid binding protein and catabolic enzymes in a migratory shorebird Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2002; 282(5): R1405 - R1413. [Abstract] [Full Text] [PDF] |
||||
![]() |
G. R. Steinberg, D. J. Dyck, J. Calles-Escandon, N. N. Tandon, J. J. F. P. Luiken, J. F. C. Glatz, and A. Bonen Chronic Leptin Administration Decreases Fatty Acid Uptake and Fatty Acid Transporters in Rat Skeletal Muscle J. Biol. Chem., March 8, 2002; 277(11): 8854 - 8860. [Abstract] [Full Text] [PDF] |
||||
![]() |
O. Matzinger, P. Schneiter, and L. Tappy Effects of fatty acids on exercise plus insulin-induced glucose utilization in trained and sedentary subjects Am J Physiol Endocrinol Metab, January 1, 2002; 282(1): E125 - E131. [Abstract] [Full Text] [PDF] |
||||
![]() |
L. P. Turcotte, J. R. Swenberger, M. Zavitz Tucker, and A. J. Yee Increased Fatty Acid Uptake and Altered Fatty Acid Metabolism in Insulin-Resistant Muscle of Obese Zucker Rats Diabetes, June 1, 2001; 50(6): 1389 - 1396. [Abstract] [Full Text] |
||||
![]() |
W. G. Campbell, S. E. Gordon, C. J. Carlson, J. S. Pattison, M. T. Hamilton, and F. W. Booth Differential global gene expression in red and white skeletal muscle Am J Physiol Cell Physiol, April 1, 2001; 280(4): C763 - C768. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. Binnert, H. A. Koistinen, G. Martin, F. Andreelli, P. Ebeling, V. A. Koivisto, M. Laville, J. Auwerx, and H. Vidal Fatty acid transport protein-1 mRNA expression in skeletal muscle and in adipose tissue in humans Am J Physiol Endocrinol Metab, November 1, 2000; 279(5): E1072 - E1079. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Bonen, D. Miskovic, M. Tonouchi, K. Lemieux, M. C. Wilson, A. Marette, and A. P. Halestrap Abundance and subcellular distribution of MCT1 and MCT4 in heart and fast-twitch skeletal muscles Am J Physiol Endocrinol Metab, June 1, 2000; 278(6): E1067 - E1077. [Abstract] [Full Text] [PDF] |
||||
![]() |
D. J. Dyck, D. Miskovic, L. Code, J. J. F. P. Luiken, and A. Bonen Endurance training increases FFA oxidation and reduces triacylglycerol utilization in contracting rat soleus Am J Physiol Endocrinol Metab, May 1, 2000; 278(5): E778 - E785. [Abstract] [Full Text] [PDF] |
||||
![]() |
G. B. McClelland, P. W. Hochachka, and J.-M. Weber Effect of high-altitude acclimation on NEFA turnover and lipid utilization during exercise in rats Am J Physiol Endocrinol Metab, December 1, 1999; 277(6): E1095 - E1102. [Abstract] [Full Text] [PDF] |
||||
![]() |
J.-A. SIMONEAU, JACQUES. H. VEERKAMP, L. P. TURCOTTE, and D. E. KELLEY Markers of capacity to utilize fatty acids in human skeletal muscle: relation to insulin resistance and obesity and effects of weight loss FASEB J, November 1, 1999; 13(14): 2051 - 2060. [Abstract] [Full Text] |
||||
![]() |
F. G. Schaap, B. Binas, H. Danneberg, G. J. van der Vusse, and J. F. C. Glatz Impaired Long-Chain Fatty Acid Utilization by Cardiac Myocytes Isolated From Mice Lacking the Heart-Type Fatty Acid Binding Protein Gene Circ. Res., August 20, 1999; 85(4): 329 - 337. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. C. Chatham, Z.-P. Gao, and J. R. Forder Impact of 1 wk of diabetes on the regulation of myocardial carbohydrate and fatty acid oxidation Am J Physiol Endocrinol Metab, August 1, 1999; 277(2): E342 - E351. [Abstract] [Full Text] [PDF] |
||||
![]() |
L. P. Turcotte, J. R. Swenberger, M. Z. Tucker, and A. J. Yee Training-induced elevation in FABPPM is associated with increased palmitate use in contracting muscle J Appl Physiol, July 1, 1999; 87(1): 285 - 293. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Bonen, David. J. Dyck, A. Ibrahimi, and N. A. Abumrad Muscle contractile activity increases fatty acid metabolism and transport and FAT/CD36 Am J Physiol Endocrinol Metab, April 1, 1999; 276(4): E642 - E649. [Abstract] [Full Text] [PDF] |
||||
![]() |
B. Kiens, T. H. M. Roemen, and G. J. van der Vusse Muscular long-chain fatty acid content during graded exercise in humans Am J Physiol Endocrinol Metab, February 1, 1999; 276(2): E352 - E357. [Abstract] [Full Text] [PDF] |
||||
![]() |
G. R. Steinberg, A. Bonen, and D. J. Dyck Fatty acid oxidation and triacylglycerol hydrolysis are enhanced after chronic leptin treatment in rats Am J Physiol Endocrinol Metab, March 1, 2002; 282(3): E593 - E600. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Tonouchi, H. Hatta, and A. Bonen Muscle contraction increases lactate transport while reducing sarcolemmal MCT4, but not MCT1 Am J Physiol Endocrinol Metab, May 1, 2002; 282(5): E1062 - E1069. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |