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1 Department of Anatomy,
The effects of epinephrine on glucose metabolism
during contractile activity and insulin stimulation were investigated
in fast-twitch (epitrochlearis) and slow-twitch (soleus) muscles from
Wistar rats. All muscles were mounted on contraction apparatuses, and
some muscles were stimulated electrically for 30 min in vitro. Glucose
uptake and glucose phosphorylation were measured with 2-[1,2-3H(N)]deoxy-D-glucose
and glucose transport with
3-O-[methyl-3H]methyl-D-glucose.
D-[1-14C]mannitol
was used to correct for extracellular space. In epitrochlearis, both
contraction and insulin increased glucose transport by threefold, and
combined they showed an additive effect. Epinephrine
(10
6 M) did not influence
glucose transport across the membrane during contractile activity or
insulin stimulation. In the absence of epinephrine, similar glucose
phosphorylation was obtained during contraction and during insulin
stimulation in epitrochlearis (~12 mmol · kg dry
wt1 · 30 min1). In the presence of
epinephrine, 9.5 ± 0.6 mmol · kg dry
wt1 · 30 min1 glucose was
phosphorylated during contraction, whereas only 2.0 ± 0.3 mmol · kg dry
wt1 · 30 min1 was phosphorylated
during insulin stimulation (P < 0.01), despite a similar glucose 6-phosphate concentration. Comparable
results were obtained in soleus. In conclusion, our data suggest that epinephrine inhibits glucose phosphorylation much less during contraction than during insulin stimulation.
glucose 6-phosphate; glucose phosphorylation; glycogen; insulin
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