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Am J Physiol Endocrinol Metab 274: E936-E945, 1998;
0193-1849/98 $5.00
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Vol. 274, Issue 5, E936-E945, May 1998

SPECIAL COMMUNICATION
Metabolite levels in human skeletal muscle and adipose tissue studied with microdialysis at low perfusion flow

H. Rosdahl1,2, K. Hamrin1, U. Ungerstedt1, and J. Henriksson1

1 Department of Physiology and Pharmacology, Karolinska Institutet, and 2 Department of Human Biology, Stockholm University College of Physical Education and Sports, S-11486 Stockholm, Sweden

To identify a perfusion flow at which the interstitial fluid completely equilibrates with the microdialysis perfusion fluid, a protocol with successively lower perfusion flows was used. A colloid was included in the perfusion fluid to make sampling possible at the lowest perfusion flows. At 0.16 µl/min, the measured metabolites had reached a complete equilibration in both tissues, and the measured concentrations of glucose, glycerol, and urea were in good agreement with expected tissue-specific levels. The glucose concentration in adipose tissue (4.98 ± 0.14 mM) was equal to that of plasma (5.07 ± 0.07 mM), whereas the concentration in muscle (4.41 ± 0.11 mM) was lower than in plasma and adipose tissue (P < 0.001). The concentration of lactate was higher (P < 0.001) in muscle (2.39 ± 0.22 mM) than in adipose tissue (1.30 ± 0.12 mM), whereas the glycerol concentration in adipose tissue (233 ± 19.7 µM) was higher (P < 0.001) than in muscle (40.8 ± 3.0 µM) and in plasma (68.7 ± 3.97 µM). The concentration of urea was equal in the two tissues. Overall, the study indicates that microdialysis at a low perfusion flow may be a tool to continuously monitor tissue interstitial concentrations.

dextran; glucose; lactate; glycerol; urea


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