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Department of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1
We examined the effects of epinephrine (25, 50, and 150 nM) on 1) basal and
insulin-stimulated 3-O-methylglucose
(3-MG) transport in perfused rat muscles and
2) GLUT-4 in skeletal muscle plasma membranes. Insulin increased glucose transport 330-600% in three types of skeletal muscle [white (WG) and red (RG) gastrocnemius and soleus (SOL)]. Glucose transport was also increased by
epinephrine (22-48%) in these muscles
(P < 0.05). In contrast, the
insulin-stimulated 3-MG transport was reduced by epinephrine in all
three types of muscles; maximal reductions were observed at 25 nM
epinephrine in WG (
25%) and RG (32.5%). A
dose-dependent decrease occurred in SOL (27% at 25 nM;
55% at 150 nM, P < 0.05).
Insulin (20 mU/ml) and epinephrine (150 nM) each translocated GLUT-4 to
the plasma membrane, and no differences in translocation were observed between insulin and epinephrine (P > 0.05). In addition, epinephrine did not inhibit insulin-stimulated
GLUT-4 translocation, and the combined epinephrine and insulin effects
on GLUT-4 translocation were not additive. The increase in surface
GLUT-4 was associated with increases in muscle cAMP concentrations, but
only when epinephrine alone was present. No relationship was evident
between muscle cAMP concentrations and surface GLUT-4 in the combined
epinephrine and insulin-stimulated muscles. These studies indicate that
epinephrine can translocate GLUT-4 while at the same time increasing
glucose transport when insulin is absent, or can inhibit glucose
transport when insulin is present.
soleus; red gastrocnemius; white gastrocnemius; adenosine 3',5'-cyclic monophosphate; perfusion
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