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Am J Physiol Endocrinol Metab 274: E527-E533, 1998;
0193-1849/98 $5.00
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Vol. 274, Issue 3, E527-E533, March 1998

Brown fat is essential for cold-induced thermogenesis but not for obesity resistance in aP2-Ucp mice

Bohumír Stefl, Alena Janovská, Zdenek Hodný, Martin Rossmeisl, Milada Horáková, Ivo Syrový, Jaroslava Bémová, Bela Bendlová, and Jan Kopecký

Faculty of Sciences, Charles University, 128 00 Prague; Institute of Endocrinology, 116 91 Prague; and Institute of Physiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czech Republic

The role of brown adipose tissue in total energy balance and cold-induced thermogenesis was studied. Mice expressing mitochondrial uncoupling protein 1 (UCP-1) from the fat-specific aP2 gene promoter (heterozygous and homozygous aP2-Ucp transgenic mice) and their nontransgenic C57BL6/J littermates were used. The transgenic animals are resistant to obesity induced by a high-fat diet, presumably due to ectopic synthesis of UCP-1 in white fat. These animals exhibited atrophy of brown adipose tissue, as indicated by smaller size of brown fat and reduction of its total UCP-1 and DNA contents. Norepinephrine-induced respiration (measured in pentobarbital sodium-anesthetized animals) was decreased proportionally to the dosage of the transgene, and the homozygous (but not heterozygous) transgenic mice exhibited a reduction in their capacity to maintain body temperature in the cold. Our results indicate that the role of brown fat in cold-induced thermogenesis cannot be substituted by increased energy expenditure in other tissues.

C57BL/6J mouse strain; uncoupling protein 1; high-fat diet; adipose tissue; mitochondria


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