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Department of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1; The Karolinska Institute, S-141 Stockholm 86, Sweden; and Department of Medicine, McMaster University Medical Centre, Hamilton, Ontario, Canada L8N 3Z5
We examined the effects of increasing a known
lactate transporter protein, monocarboxylate transporter 1 (MCT1), on
lactate extrusion from human skeletal muscle during exercise. Before
and after short-term bicycle ergometry training [2 h/day, 7 days
at 65% maximal oxygen consumption
(
O2 max)],
subjects (n = 7) completed a
continuous bicycle ergometer ride at 30%
O2 max (15 min), 60%
O2 max (15 min), and
75% O2 max (15 min).
Muscle biopsy samples (vastus lateralis) and arterial and femoral
venous blood samples were obtained before exercise and at the end of
each workload. After 7 days of training the MCT1 content in muscle was
increased (+18%; P < 0.05). The concentrations of both muscle lactate and femoral venous
lactate were reduced during exercise
(P < 0.05) that was performed after
training. High correlations were observed between muscle lactate and
venous lactate before training (r = 0.92, P < 0.05) and after training
(r = 0.85, P < 0.05), but the slopes of the
regression lines between these variables differed markedly. Before
training, the slope was 0.12 ± 0.01 mM
lactate · mmol
lactate
1 · kg
muscle dry wt1, and this
was increased by 33% after training to 0.18 ± 0.02 mM
lactate · mmol
lactate1 · kg
muscle dry wt1. This
indicated that after training the femoral venous lactate concentrations
were increased for a given amount of muscle lactate. These results
suggest that lactate extrusion from exercising muscles is increased
after training, and this may be associated with the increase in
skeletal muscle MCT1.
glycogen; exercise; muscle lactate; femoral venous lactate; arteriovenous difference
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