AJP - Endo Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 273: E922-E931, 1997;
0193-1849/97 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rebsamen, M. C.
Right arrow Articles by Lang, U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rebsamen, M. C.
Right arrow Articles by Lang, U.
Vol. 273, Issue 5, E922-E931, November 1997

Role of cAMP and calcium influx in endothelin-1-induced ANP release in rat cardiomyocytes

M. C. Rebsamen1, D. J. Church2, D. Morabito1, M. B. Vallotton1, and U. Lang1

1 Division of Endocrinology and Diabetology, University Hospital, and 2 Geneva Biomedical Research Institute, CH-1211 Geneva 14, Switzerland

The mechanism of endothelin-1 (ET-1)-induced atrial natriuretic peptide (ANP) release was studied in neonatal rat ventricular cardiomyocytes. These cells expressed a single high-affinity class of ETA receptor (dissociation constant = 54 ± 18 pM, n = 3), but no ETB receptors. Incubation of cardiomyocytes with ET-1 led to concentration-dependent ANP release and prostacyclin production. ET-1-induced ANP release was affected by neither protein kinase C (PKC) inhibition or downregulation nor by cyclooxygenase inhibition, indicating that ET-1-stimulated ANP secretion is not a PKC-mediated, prostaglandin-dependent process. Furthermore, ET-1 significantly stimulated adenosine 3',5'-cyclic monophosphate (cAMP) production and increased cytosolic calcium concentration in these preparations. Both ET-1-induced calcium influx and ANP release were decreased by the cAMP antagonist Rp-cAMPS, the Rp diastereoisomer of cAMP. Moreover, ET-1-induced ANP secretion was strongly inhibited in the presence of nifedipine as well as in the absence of extracellular calcium. Thus our results suggest that ET-1 stimulates ANP release in ventricular cardiomyocytes via an ETA receptor-mediated pathway involving cAMP formation and activation of a nifedipine-sensitive calcium channel.

endothelin-1; receptor; prostacyclin formation; protein kinase C downregulation; adenosine 3',5'-cyclic monophosphate antagonist; nifedipine-sensitive calcium channel; signal transduction; atrial natriuretic peptide


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. Baron, D. Monnier, A. Roatti, and A. J. Baertschi
Pituitary adenylate cyclase-activating polypeptide activates KATP current in rat atrial myocytes
Am J Physiol Heart Circ Physiol, March 1, 2001; 280(3): H1058 - H1065.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. M. Eble, J. B. Strait, G. Govindarajan, J. Lou, K. L. Byron, and A. M. Samarel
Endothelin-induced cardiac myocyte hypertrophy: role for focal adhesion kinase
Am J Physiol Heart Circ Physiol, May 1, 2000; 278(5): H1695 - H1707.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
B. Hocher, I. George, J. Rebstock, A. Bauch, A. Schwarz, H.-H. Neumayer, and C. Bauer
Endothelin System–Dependent Cardiac Remodeling in Renovascular Hypertension
Hypertension, March 1, 1999; 33(3): 816 - 822.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. O. Dulin, J. Niu, D. D. Browning, R. D. Ye, and T. Voyno-Yasenetskaya
Cyclic AMP-independent Activation of Protein Kinase A by Vasoactive Peptides
J. Biol. Chem., June 8, 2001; 276(24): 20827 - 20830.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online