AJP - Endo Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 273: E859-E867, 1997;
0193-1849/97 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hettiarachchi, M.
Right arrow Articles by Kraegen, E. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hettiarachchi, M.
Right arrow Articles by Kraegen, E. W.
Vol. 273, Issue 5, E859-E867, November 1997

Rat amylin-(8---37) enhances insulin action and alters lipid metabolism in normal and insulin-resistant rats

M. Hettiarachchi1, S. Chalkley1, S. M. Furler1, Y.-S. Choong2, M. Heller2, G. J. S. Cooper2,3, and E. W. Kraegen1

1 Garvan Institute of Medical Research, Darlinghurst, New South Wales 2010, Australia; and 2 School of Biological Sciences and 3 Department of Medicine, School of Medicine, University of Auckland, Auckland 1, New Zealand

To clarify roles of amylin, we investigated metabolic responses to rat amylin-(8---37), a specific amylin antagonist, in normal and insulin-resistant, human growth hormone (hGH)-infused rats. Fasting conscious rats were infused with saline or hGH, each with and without amylin-(8---37) (0.125 µmol/h), over 5.75 h. At 3.75 h, a hyperinsulinemic (100 mU/l) clamp with bolus 2-deoxy-D-[3H]glucose and [14C]glucose was started. hGH infusion led to prompt (2- to 3-fold) basal hyperamylinemia (P < 0.02) and hyperinsulinemia. Amylin-(8---37) reduced plasma insulin (P < 0.001) and enhanced several measures of whole body and muscle insulin sensitivity (P < 0.05) in both saline- and hGH-infused rats. Amylin-(8---37) corrected hGH-induced liver insulin resistance, increased basal plasma triglycerides and lowered plasma nonesterified fatty acids in both groups, and reduced muscle triglyceride and total long-chain acyl-CoA content in saline-treated rats (P < 0.05). In isolated soleus muscle, amylin-(8---37) blocked amylin-induced inhibition of glycogen synthesis but had no effect in the absence of amylin. Thus 1) hyperamylinemia accompanies insulin resistance induced by hGH infusion; 2) amylin-(8---37) increases whole body and muscle insulin sensitivity and consistently reduces basal insulin levels in normal and hGH-induced insulin-resistant rats; and 3) amylin-(8---37) elicits a significant alteration of in vivo lipid metabolism. These findings support a role of amylin in modulating insulin action and suggest that this could be mediated by effects on lipid metabolism.

human growth hormone; euglycemic clamp; muscle; liver; triglycerides; long-chain acyl-CoA


This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
D. Arsenijevic, E. Gallmann, W. Moses, T. Lutz, C. Erlanson-Albertsson, and W. Langhans
Enterostatin decreases postprandial pancreatic UCP2 mRNA levels and increases plasma insulin and amylin
Am J Physiol Endocrinol Metab, July 1, 2005; 289(1): E40 - E45.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
Y. Wang, A. Xu, J. Ye, E. W. Kraegen, C. A. Tse, and G. J.S. Cooper
Alteration in Phosphorylation of P20 Is Associated With Insulin Resistance
Diabetes, August 1, 2001; 50(8): 1821 - 1827.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J.-M. Ye, M. Lim-Fraser, G. J. Cooney, G. J. S. Cooper, M. A. Iglesias, D. G. Watson, B. Choong, and E. W. Kraegen
Evidence that amylin stimulates lipolysis in vivo: a possible mediator of induced insulin resistance
Am J Physiol Endocrinol Metab, April 1, 2001; 280(4): E562 - E569.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online