| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Endocrine Research Unit, Mayo Clinic and Foundation, Rochester, Minnesota 55905; and 2 Department of Medicine, University of Vermont, Burlington, Vermont 05405
A decline in muscle mass and contractile function are prominent features of the sarcopenia of old age. Because myosin heavy chain is an important contractile protein, it was hypothesized that synthesis of this protein decreases in sarcopenia. The fractional synthesis rate of myosin heavy chain was measured simultaneously with rates of mixed muscle and sarcoplasmic proteins from the increment of [13C]leucine in these proteins purified from serial needle biopsy samples taken from 24 subjects (age: from 20 to 92 yr) during a primed continuous infusion of L-[1-13C]leucine. A decline in synthesis rate of mixed muscle protein (P < 0.01) and whole body protein (P < 0.01) was observed from young to middle age with no further change with advancing age. An age-related decline of myosin heavy-chain synthesis rate was also observed (P < 0.01), with progressive decline occurring from young, through middle, to old age. However, sarcoplasmic protein synthesis did not decline with age. Myosin heavy-chain synthesis rate was correlated with measures of muscle strength (P < 0.05), circulating insulin-like growth factor I (P < 0.01), and dehydroepiandrosterone sulfate (P < 0.05) in men and women and free testosterone levels in men (P < 0.01). A decline in the synthesis rate of myosin heavy chain implies a decreased ability to remodel this important muscle contractile protein and likely contributes to the declining muscle mass and contractile function in the elderly.
protein synthesis; sarcopenia; contractile function; mass spectrometry; stable isotope
This article has been cited by other articles:
|
|
 |
|
  R. A. Dennis, B. Przybyla, C. Gurley, P. M. Kortebein, P. Simpson, D. H. Sullivan, and C. A. Peterson Aging alters gene expression of growth and remodeling factors in human skeletal muscle both at rest and in response to acute resistance exercise Physiol Genomics, February 19, 2008; 32(3): 393 - 400. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Prochniewicz, D. A. Lowe, D. J. Spakowicz, L. Higgins, K. O'Conor, L. V. Thompson, D. A. Ferrington, and D. D. Thomas Functional, structural, and chemical changes in myosin associated with hydrogen peroxide treatment of skeletal muscle fibers Am J Physiol Cell Physiol, February 1, 2008; 294(2): C613 - C626. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Haus, J. A. Carrithers, S. W. Trappe, and T. A. Trappe Collagen, cross-linking, and advanced glycation end products in aging human skeletal muscle J Appl Physiol, December 1, 2007; 103(6): 2068 - 2076. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Fujita, B. B. Rasmussen, J. A. Bell, J. G. Cadenas, and E. Volpi Basal muscle intracellular amino acid kinetics in women and men Am J Physiol Endocrinol Metab, January 1, 2007; 292(1): E77 - E83. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. V. Thompson, D. Durand, N. A. Fugere, and D. A. Ferrington Myosin and actin expression and oxidation in aging muscle J Appl Physiol, December 1, 2006; 101(6): 1581 - 1587. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. R Wolfe The underappreciated role of muscle in health and disease. Am. J. Clinical Nutrition, September 1, 2006; 84(3): 475 - 482. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Korhonen, A. Cristea, M. Alen, K. Hakkinen, S. Sipila, A. Mero, J. T. Viitasalo, L. Larsson, and H. Suominen Aging, muscle fiber type, and contractile function in sprint-trained athletes J Appl Physiol, September 1, 2006; 101(3): 906 - 917. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Kosek, J.-s. Kim, J. K. Petrella, J. M. Cross, and M. M. Bamman Efficacy of 3 days/wk resistance training on myofiber hypertrophy and myogenic mechanisms in young vs. older adults J Appl Physiol, August 1, 2006; 101(2): 531 - 544. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Funai, J. D. Parkington, S. Carambula, and R. A. Fielding Age-associated decrease in contraction-induced activation of downstream targets of Akt/mTor signaling in skeletal muscle Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2006; 290(4): R1080 - R1086. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Balagopal, R. Olney, D. Darmaun, E. Mougey, M. Dokler, G. Sieck, and D. Hammond Oxandrolone enhances skeletal muscle myosin synthesis and alters global gene expression profile in Duchenne muscular dystrophy Am J Physiol Endocrinol Metab, March 1, 2006; 290(3): E530 - E539. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. F. Miller, M. Hansen, J. L. Olesen, A. Flyvbjerg, P. Schwarz, J. A. Babraj, K. Smith, M. J. Rennie, and M. Kjaer No effect of menstrual cycle on myofibrillar and connective tissue protein synthesis in contracting skeletal muscle Am J Physiol Endocrinol Metab, January 1, 2006; 290(1): E163 - E168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. R. Short, J. L. Vittone, M. L. Bigelow, D. N. Proctor, J. M. Coenen-Schimke, P. Rys, and K. S. Nair Changes in myosin heavy chain mRNA and protein expression in human skeletal muscle with age and endurance exercise training J Appl Physiol, July 1, 2005; 99(1): 95 - 102. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Canepari, R. Rossi, M. A. Pellegrino, R. W. Orrell, M. Cobbold, S. Harridge, and R. Bottinelli Effects of resistance training on myosin function studied by the in vitro motility assay in young and older men J Appl Physiol, June 1, 2005; 98(6): 2390 - 2395. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Toth, D. E. Matthews, R. P. Tracy, and M. J. Previs Age-related differences in skeletal muscle protein synthesis: relation to markers of immune activation Am J Physiol Endocrinol Metab, May 1, 2005; 288(5): E883 - E891. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Kimball, J. P. O'Malley, J. C. Anthony, S. J. Crozier, and L. S. Jefferson Assessment of biomarkers of protein anabolism in skeletal muscle during the life span of the rat: sarcopenia despite elevated protein synthesis Am J Physiol Endocrinol Metab, October 1, 2004; 287(4): E772 - E780. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Parkington, N. K. LeBrasseur, A. P. Siebert, and R. A. Fielding Contraction-mediated mTOR, p70S6k, and ERK1/2 phosphorylation in aged skeletal muscle J Appl Physiol, July 1, 2004; 97(1): 243 - 248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Paddon-Jones, M. Sheffield-Moore, X.-J. Zhang, E. Volpi, S. E. Wolf, A. Aarsland, A. A. Ferrando, and R. R. Wolfe Amino acid ingestion improves muscle protein synthesis in the young and elderly Am J Physiol Endocrinol Metab, March 1, 2004; 286(3): E321 - E328. [Abstract] [Full Text]
|
|
|
|
|
|
K. R. Short, J. L. Vittone, M. L. Bigelow, D. N. Proctor, and K. S. Nair Age and aerobic exercise training effects on whole body and muscle protein metabolism Am J Physiol Endocrinol Metab, January 1, 2004; 286(1): E92 - E101. [Abstract] [Full Text]
|
|
|
|
|
|
F. Lauretani, C. R. Russo, S. Bandinelli, B. Bartali, C. Cavazzini, A. Di Iorio, A. M. Corsi, T. Rantanen, J. M. Guralnik, and L. Ferrucci Age-associated changes in skeletal muscles and their effect on mobility: an operational diagnosis of sarcopenia J Appl Physiol, November 1, 2003; 95(5): 1851 - 1860. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Doherty Invited Review: Aging and sarcopenia J Appl Physiol, October 1, 2003; 95(4): 1717 - 1727. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Marcell Review Article: Sarcopenia: Causes, Consequences, and Preventions J. Gerontol. A Biol. Sci. Med. Sci., October 1, 2003; 58(10): M911 - 916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Ramamurthy, A. D. Jones, and L. Larsson Glutathione reverses early effects of glycation on myosin function Am J Physiol Cell Physiol, August 1, 2003; 285(2): C419 - C424. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. O. Marx, W. J. Kraemer, B. C. Nindl, and L. Larsson Effects of Aging on Human Skeletal Muscle Myosin Heavy-Chain mRNA Content and Protein Isoform Expression J. Gerontol. A Biol. Sci. Med. Sci., June 1, 2002; 57(6): B232 - 238. [Abstract] [Full Text]
|
|
|
|
|
|
T. H. Reynolds IV, K. M. Krajewski, L. M. Larkin, P. Reid, J. B. Halter, M. A. Supiano, and D. R. Dengel Effect of Age on Skeletal Muscle Proteolysis in Extensor Digitorum Longus Muscles of B6C3F1 Mice J. Gerontol. A Biol. Sci. Med. Sci., May 1, 2002; 57(5): B198 - 201. [Abstract] [Full Text]
|
|
|
|
 |
|
 N. da Costa, R. Blackley, H. Alzuherri, and K.-C. Chang Quantifying the Temporospatial Expression of Postnatal Porcine Skeletal Myosin Heavy Chain Genes J. Histochem. Cytochem., March 1, 2002; 50(3): 353 - 364. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Dardevet, C. Sornet, G. Bayle, J. Prugnaud, C. Pouyet, and J. Grizard Postprandial Stimulation of Muscle Protein Synthesis in Old Rats Can Be Restored by a Leucine-Supplemented Meal J. Nutr., January 1, 2002; 132(1): 95 - 100. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Boirie, K. R. Short, B. Ahlman, M. Charlton, and K. S. Nair Tissue-Specific Regulation of Mitochondrial and Cytoplasmic Protein Synthesis Rates by Insulin Diabetes, December 1, 2001; 50(12): 2652 - 2658. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. RAMAMURTHY, P. HOOK, A. D. JONES, and L. LARSSON Changes in myosin structure and function in response to glycation FASEB J, November 1, 2001; 15(13): 2415 - 2422. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Parise, S. Mihic, D. MacLennan, K. E. Yarasheski, and M. A. Tarnopolsky Effects of acute creatine monohydrate supplementation on leucine kinetics and mixed-muscle protein synthesis J Appl Physiol, September 1, 2001; 91(3): 1041 - 1047. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.L. Ploutz-Snyder, E.L. Giamis, M. Formikell, and A.E. Rosenbaum Resistance Training Reduces Susceptibility to Eccentric Exercise-Induced Muscle Dysfunction in Older Women J. Gerontol. A Biol. Sci. Med. Sci., September 1, 2001; 56(9): B384 - 390. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Jubrias, P. C. Esselman, L. B. Price, M. E. Cress, and K. E. Conley Large energetic adaptations of elderly muscle to resistance and endurance training J Appl Physiol, May 1, 2001; 90(5): 1663 - 1670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Ahlman, M. Charlton, A. Fu, C. Berg, P. O’Brien, and K. S. Nair Insulin's Effect on Synthesis Rates of Liver Proteins: A Swine Model Comparing Various Precursors of Protein Synthesis Diabetes, May 1, 2001; 50(5): 947 - 954. [Abstract] [Full Text]
|
|
|
|
|
|
P. Hook, V. Sriramoju, and L. Larsson Effects of aging on actin sliding speed on myosin from single skeletal muscle cells of mice, rats, and humans Am J Physiol Cell Physiol, April 1, 2001; 280(4): C782 - C788. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Balagopal, J. C. Schimke, P. Ades, D. Adey, and K. S. Nair Age effect on transcript levels and synthesis rate of muscle MHC and response to resistance exercise Am J Physiol Endocrinol Metab, February 1, 2001; 280(2): E203 - E208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Roubenoff and V. A. Hughes Sarcopenia: Current Concepts J. Gerontol. A Biol. Sci. Med. Sci., December 1, 2000; 55(12): 716M - 724. [Abstract] [Full Text]
|
|
|
|
|
|
D. Danieli-Betto, E. Germinario, A. Esposito, D. Biral, and R. Betto Effects of fatigue on sarcoplasmic reticulum and myofibrillar properties of rat single muscle fibers J Appl Physiol, September 1, 2000; 89(3): 891 - 898. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. ZAINAL, T. D. OBERLEY, D. B. ALLISON, L. I. SZWEDA, and R. WEINDRUCH Caloric restriction of rhesus monkeys lowers oxidative damage in skeletal muscle FASEB J, September 1, 2000; 14(12): 1825 - 1836. [Abstract] [Full Text]
|
|
|
|
|
|
W. R. Frontera, D. Suh, L. S. Krivickas, V. A. Hughes, R. Goldstein, and R. Roubenoff Skeletal muscle fiber quality in older men and women Am J Physiol Cell Physiol, September 1, 2000; 279(3): C611 - C618. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Gallagher, E. Ruts, M. Visser, S. Heshka, R. N. Baumgartner, J. Wang, R. N. Pierson, F. X. Pi-Sunyer, and S. B. Heymsfield Weight stability masks sarcopenia in elderly men and women Am J Physiol Endocrinol Metab, August 1, 2000; 279(2): E366 - E375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Tamaki, S. Uchiyama, Y. Uchiyama, A. Akatsuka, S. Yoshimura, R. R. Roy, and V. R. Edgerton Limited myogenic response to a single bout of weight-lifting exercise in old rats Am J Physiol Cell Physiol, June 1, 2000; 278(6): C1143 - C1152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Hasten, J. Pak-Loduca, K. A. Obert, and K. E. Yarasheski Resistance exercise acutely increases MHC and mixed muscle protein synthesis rates in 78-84 and 23-32 yr olds Am J Physiol Endocrinol Metab, April 1, 2000; 278(4): E620 - E626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Adey, R. Kumar, J. T. McCarthy, and K. S. Nair Reduced synthesis of muscle proteins in chronic renal failure Am J Physiol Endocrinol Metab, February 1, 2000; 278(2): E219 - E225. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Volpi, B. Mittendorfer, S. E. Wolf, and R. R. Wolfe Oral amino acids stimulate muscle protein anabolism in the elderly despite higher first-pass splanchnic extraction Am J Physiol Endocrinol Metab, September 1, 1999; 277(3): E513 - E520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D Starling, P. A Ades, and E. T Poehlman Physical activity, protein intake, and appendicular skeletal muscle mass in older men Am. J. Clinical Nutrition, July 1, 1999; 70(1): 91 - 96. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Yarasheski, J. Pak-Loduca, D. L. Hasten, K. A. Obert, M. B. Brown, and D. R. Sinacore Resistance exercise training increases mixed muscle protein synthesis rate in frail women and men >= 76 yr old Am J Physiol Endocrinol Metab, July 1, 1999; 277(1): E118 - E125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-A. Arnal, L. Mosoni, Y. Boirie, M.-L. Houlier, L. Morin, E. Verdier, P. Ritz, J.-M. Antoine, J. Prugnaud, B. Beaufrere, et al. Protein pulse feeding improves protein retention in elderly women Am. J. Clinical Nutrition, June 1, 1999; 69(6): 1202 - 1208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Fu and K. S. Nair Age effect on fibrinogen and albumin synthesis in humans Am J Physiol Endocrinol Metab, December 1, 1998; 275(6): E1023 - E1030. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. A. El-Harake, M. A. Furman, B. Cook, K. S. Nair, J. Kukowski, and I. G. Brodsky Measurement of dermal collagen synthesis rate in vivo in humans Am J Physiol Endocrinol Metab, April 1, 1998; 274(4): E586 - E591. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Germinario, A. Esposito, A. Megighian, M. Midrio, D. Biral, R. Betto, and D. Danieli-Betto Early changes of type 2B fibers after denervation of rat EDL skeletal muscle J Appl Physiol, May 1, 2002; 92(5): 2045 - 2052. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Balagopal, S. Sweeten, and N. Mauras Increased synthesis rate of fibrinogen as a basis for its elevated plasma levels in obese female adolescents Am J Physiol Endocrinol Metab, April 1, 2002; 282(4): E899 - E904. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
