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Am J Physiol Endocrinol Metab 273: E543-E548, 1997;
0193-1849/97 $5.00
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AJP - Endocrinology and Metabolism, Vol 273, Issue 3 E543-E548, Copyright © 1997 by American Physiological Society

ARTICLES

Fructose transport and GLUT-5 protein in human sarcolemmal vesicles

S. Kristiansen, F. Darakhshan, E. A. Richter and H. S. Hundal
Copenhagen Muscle Research Centre, August Krogh Institute, University of Copenhagen, Denmark.

Sarcolemmal vesicles were produced from human skeletal muscle biopsy material obtained at rest and immediately after maximal dynamic exercise (100% maximal O2 uptake) for analysis of fructose transport and hexose transporter (GLUT-5) protein concentration. Human sarcolemmal vesicles displayed a time-dependent uptake of D-fructose that displayed saturable Michaelis-Menten type kinetics (maximal transport 477 +/- 37 pmol.min-1.mg protein-1; half-maximal concentration constant 8.3 +/- 1.2 mM). At a hexose concentration of 5 mM, vesicle transport rate was eight times faster for glucose than for fructose. Preincubation of human muscle vesicles with 35 microM cytochalasin B before the uptake assay resulted in > 95% inhibition in D-glucose uptake, whereas transport of D-fructose was unaffected. Sarcolemmal vesicles prepared from exercised human muscle showed a significant increase (49%) in vesicle GLUT-4 content (P < 0.03, n = 10), which accounts for the increase in vesicle glucose transport that we have recently reported [S. Kristiansen, M. Hargreaves, and E.A. Richter. Am. J. Physiol. 270 (Endocrinol. Metab. 33): E197-E201, 1996]. In contrast, exercise did not increase the vesicle GLUT-5 protein content or induce changes in vesicle fructose transport activity. In conclusion, we propose that fructose transport into human skeletal muscle occurs via a mechanism distinct from that utilized by glucose on the basis of differences in sensitivity to cytochalasin B and responsiveness to exercise. Furthermore, our findings signify that uptake of fructose in human skeletal muscle is mediated by the GLUT-5 transporter.


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