| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Endocrinology and Metabolism, Vol 273, Issue 2 E268-E275, Copyright © 1997 by American Physiological Society
ARTICLES |
E. F. Coyle, A. E. Jeukendrup, A. J. Wagenmakers and W. H. Saris
Department of Human Biology, University of Limburg, Maastricht, The Netherlands.
We determined whether increased glycolytic flux from hyperglycemia and hyperinsulinemia directly reduces fatty acid oxidation during exercise. Fatty acid oxidation rates were measured during constant-rate intravenous infusion of trace amounts of a long-chain fatty acid ([1-13C]palmitate; Pal) vs. a medium-chain fatty acid ([1-13C]octanoate; Oct). Six endurance-trained men cycled for 40 min at 50% of maximal O2 uptake 1) after an overnight fast ("fasting") and 2) after ingestion of 1.4 g/kg of glucose at 60 min and again 10 min before exercise (Glc). Glc caused hyperinsulinemia, a preexercise blood glucose of 6 mM, and a 34% reduction in total fat oxidation during exercise due to an approximately equal reduction in oxidation of plasma-free fatty acids (FFA) and intramuscular triglycerides (all P < 0.05). Oxidation of Pal was significantly reduced during Glc compared with fast (i.e., 70.0 +/- 4.1 vs. 86.0 +/- 1.9% of tracer infusion rate; P < 0.05). However, Glc had no effect on Oct oxidation, which is apparently not limited by mitochondrial transport. Furthermore, Glc reduced plasma FFA appearance 36% (P < 0.05), indicating a coordination of effects on adipose tissue and muscle. In summary, substrate oxidation during exercise can be regulated by increased glycolytic flux that is accompanied by a direct inhibition of long-chain fatty acid oxidation. These observations indicate that carbohydrate availability can directly regulate fat oxidation during exercise.
This article has been cited by other articles:
|
|
 |
|
  F. B. Stephens, D. Constantin-Teodosiu, and P. L. Greenhaff New insights concerning the role of carnitine in the regulation of fuel metabolism in skeletal muscle J. Physiol., June 1, 2007; 581(2): 431 - 444. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Stellingwerff, H. Boon, R. A. M. Jonkers, J. M. Senden, L. L. Spriet, R. Koopman, and L. J. C. van Loon Significant intramyocellular lipid use during prolonged cycling in endurance-trained males as assessed by three different methodologies Am J Physiol Endocrinol Metab, June 1, 2007; 292(6): E1715 - E1723. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Shibakusa, W. Mizunoya, Y. Okabe, S. Matsumura, Y. Iwaki, A. Okuno, K. Shibata, K. Inoue, and T. Fushiki Transforming growth factor-beta in the brain is activated by exercise and increases mobilization of fat-related energy substrates in rats Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2007; 292(5): R1851 - R1861. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. De Bock, W. Derave, M. Ramaekers, E. A. Richter, and P. Hespel Fiber type-specific muscle glycogen sparing due to carbohydrate intake before and during exercise J Appl Physiol, January 1, 2007; 102(1): 183 - 188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ishikawa, W. Mizunoya, T. Shibakusa, K. Inoue, and T. Fushiki Transforming growth factor-beta in the brain regulates fat metabolism during endurance exercise Am J Physiol Endocrinol Metab, December 1, 2006; 291(6): E1151 - E1159. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Salinari, A. Bertuzzi, A. Gandolfi, A. V. Greco, A. Scarfone, M. Manco, and G. Mingrone Dodecanedioic acid overcomes metabolic inflexibility in type 2 diabetic subjects Am J Physiol Endocrinol Metab, November 1, 2006; 291(5): E1051 - E1058. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Kiens Skeletal Muscle Lipid Metabolism in Exercise and Insulin Resistance Physiol Rev, January 1, 2006; 86(1): 205 - 243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Civitarese, M. K. C. Hesselink, A. P. Russell, E. Ravussin, and P. Schrauwen Glucose ingestion during exercise blunts exercise-induced gene expression of skeletal muscle fat oxidative genes Am J Physiol Endocrinol Metab, December 1, 2005; 289(6): E1023 - E1029. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Yeo, R. L. P. G. Jentjens, G. A. Wallis, and A. E. Jeukendrup Caffeine increases exogenous carbohydrate oxidation during exercise J Appl Physiol, September 1, 2005; 99(3): 844 - 850. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-L. Wee, C. Williams, K. Tsintzas, and L. Boobis Ingestion of a high-glycemic index meal increases muscle glycogen storage at rest but augments its utilization during subsequent exercise J Appl Physiol, August 1, 2005; 99(2): 707 - 714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Venables, J. Achten, and A. E. Jeukendrup Determinants of fat oxidation during exercise in healthy men and women: a cross-sectional study J Appl Physiol, January 1, 2005; 98(1): 160 - 167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. C. van Loon Use of intramuscular triacylglycerol as a substrate source during exercise in humans J Appl Physiol, October 1, 2004; 97(4): 1170 - 1187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Roepstorff, B. Vistisen, K. Roepstorff, and B. Kiens Regulation of plasma long-chain fatty acid oxidation in relation to uptake in human skeletal muscle during exercise Am J Physiol Endocrinol Metab, October 1, 2004; 287(4): E696 - E705. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Arkinstall, R. J. Tunstall, D. Cameron-Smith, and J. A. Hawley Regulation of metabolic genes in human skeletal muscle by short-term exercise and diet manipulation Am J Physiol Endocrinol Metab, July 1, 2004; 287(1): E25 - E31. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Watt, P. Krustrup, N. H. Secher, B. Saltin, B. K. Pedersen, and M. A. Febbraio Glucose ingestion blunts hormone-sensitive lipase activity in contracting human skeletal muscle Am J Physiol Endocrinol Metab, January 1, 2004; 286(1): E144 - E150. [Abstract] [Full Text]
|
|
|
|
|
|
B. Vistisen, L. Nybo, X. Xu, C.-E. Hoy, and B. Kiens Minor amounts of plasma medium-chain fatty acids and no improved time trial performance after consuming lipids J Appl Physiol, December 1, 2003; 95(6): 2434 - 2443. [Abstract] [Full Text]
|
|
|
|
|
|
M. E. Walton, D. Ebert, and R. G. Haller Octanoate oxidation measured by 13C-NMR spectroscopy in rat skeletal muscle, heart, and liver J Appl Physiol, November 1, 2003; 95(5): 1908 - 1916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. F. Bernard, J. Orvoine, and R. Groscolas Glucose regulates lipid metabolism in fasting king penguins Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2003; 285(2): R313 - R320. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Watt, G. J. F. Heigenhauser, and L. L. Spriet Intramuscular triacylglycerol utilization in human skeletal muscle during exercise: is there a controversy? J Appl Physiol, October 1, 2002; 93(4): 1185 - 1195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Jose-Cunilleras, K. W. Hinchcliff, R. A. Sams, S. T. Devor, and J. K. Linderman Glycemic index of a meal fed before exercise alters substrate use and glucose flux in exercising horses J Appl Physiol, January 1, 2002; 92(1): 117 - 128. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Schrauwen, M. K. C. Hesselink, I. Vaartjes, E. Kornips, W. H. M. Saris, J.-P. Giacobino, and A. Russell Effect of acute exercise on uncoupling protein 3 is a fat metabolism-mediated effect Am J Physiol Endocrinol Metab, January 1, 2002; 282(1): E11 - E17. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. P. Turcotte, J. R. Swenberger, and A. J. Yee High carbohydrate availability increases LCFA uptake and decreases LCFA oxidation in perfused muscle Am J Physiol Endocrinol Metab, January 1, 2002; 282(1): E177 - E183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kjar, F. Dela, F. B. Sorensen, N. H. Secher, J. Bangsbo, T. Mohr, and H. Galbo Fatty acid kinetics and carbohydrate metabolism during electrical exercise in spinal cord-injured humans Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2001; 281(5): R1492 - R1498. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Mora-Rodriguez, B. J. Hodgkinson, L. O. Byerley, and E. F. Coyle Effects of {beta}-adrenergic receptor stimulation and blockade on substrate metabolism during submaximal exercise Am J Physiol Endocrinol Metab, May 1, 2001; 280(5): E752 - E760. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. F. Coyle, A. E. Jeukendrup, M. C. Oseto, B. J. Hodgkinson, and T. W. Zderic Low-fat diet alters intramuscular substrates and reduces lipolysis and fat oxidation during exercise Am J Physiol Endocrinol Metab, March 1, 2001; 280(3): E391 - E398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Horowitz and S. Klein Oxidation of nonplasma fatty acids during exercise is increased in women with abdominal obesity J Appl Physiol, December 1, 2000; 89(6): 2276 - 2282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Geor, K. W. Hinchcliff, and R. A. Sams beta -Adrenergic blockade augments glucose utilization in horses during graded exercise J Appl Physiol, September 1, 2000; 89(3): 1086 - 1098. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. F Coyle Physical activity as a metabolic stressor Am. J. Clinical Nutrition, August 1, 2000; 72(2): 512S - 520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Geor, K. W. Hinchcliff, and R. A. Sams Glucose infusion attenuates endogenous glucose production and enhances glucose use of horses during exercise J Appl Physiol, May 1, 2000; 88(5): 1765 - 1776. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Geor, K. W. Hinchcliff, L. J. McCutcheon, and R. A. Sams Epinephrine inhibits exogenous glucose utilization in exercising horses J Appl Physiol, May 1, 2000; 88(5): 1777 - 1790. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Mora-Rodriguez and E. F. Coyle Effects of plasma epinephrine on fat metabolism during exercise: interactions with exercise intensity Am J Physiol Endocrinol Metab, April 1, 2000; 278(4): E669 - E676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. C. Starritt, R. A. Howlett, G. J. F. Heigenhauser, and L. L. Spriet Sensitivity of CPT I to malonyl-CoA in trained and untrained human skeletal muscle Am J Physiol Endocrinol Metab, March 1, 2000; 278(3): E462 - E468. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Horowitz, R. Mora-Rodriguez, L. O. Byerley, and E. F. Coyle Preexercise medium-chain triglyceride ingestion does not alter muscle glycogen use during exercise J Appl Physiol, January 1, 2000; 88(1): 219 - 225. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. C. van Loon, A. E. Jeukendrup, W. H. M. Saris, and A. J. M. Wagenmakers Effect of training status on fuel selection during submaximal exercise with glucose ingestion J Appl Physiol, October 1, 1999; 87(4): 1413 - 1420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Dionne, S. Van Vugt, and A. Tremblay Postexercise macronutrient oxidation: a factor dependent on postexercise macronutrient intake Am. J. Clinical Nutrition, May 1, 1999; 69(5): 927 - 930. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Horowitz, R. Mora-Rodriguez, L. O. Byerley, and E. F. Coyle Substrate metabolism when subjects are fed carbohydrate during exercise Am J Physiol Endocrinol Metab, May 1, 1999; 276(5): E828 - E835. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Jeukendrup, A. J. M. Wagenmakers, J. H. C. H. Stegen, A. P. Gijsen, F. Brouns, and W. H. M. Saris Carbohydrate ingestion can completely suppress endogenous glucose production during exercise Am J Physiol Endocrinol Metab, April 1, 1999; 276(4): E672 - E683. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. C. Bergman and G. A. Brooks Respiratory gas-exchange ratios during graded exercise in fed and fasted trained and untrained men J Appl Physiol, February 1, 1999; 86(2): 479 - 487. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sial, A. R. Coggan, R. C. Hickner, and S. Klein Training-induced alterations in fat and carbohydrate metabolism during exercise in elderly subjects Am J Physiol Endocrinol Metab, May 1, 1998; 274(5): E785 - E790. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Sidossis, R. R. Wolfe, and A. R. Coggan Regulation of fatty acid oxidation in untrained vs. trained men during exercise Am J Physiol Endocrinol Metab, March 1, 1998; 274(3): E510 - E515. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Horowitz, R. Mora-Rodriguez, L. O. Byerley, and E. F. Coyle Lipolytic suppression following carbohydrate ingestion limits fat oxidation during exercise Am J Physiol Endocrinol Metab, October 1, 1997; 273(4): E768 - E775. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
