AJP - Endo Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 272: E1016-E1022, 1997;
0193-1849/97 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shi, J.
Right arrow Articles by Simpkins, J. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shi, J.
Right arrow Articles by Simpkins, J. W.

AJP - Endocrinology and Metabolism, Vol 272, Issue 6 E1016-E1022, Copyright © 1997 by American Physiological Society

ARTICLES

17 beta-Estradiol modulation of glucose transporter 1 expression in blood-brain barrier

J. Shi and J. W. Simpkins
Center for the Neurobiology of Aging, College of Pharmacy, University of Florida, Gainesville 32610, USA.

The present study was designed to evaluate 17 beta-estradiol (E2) modulation of glucose transporter 1 (GLUT-1) protein and mRNA expression in blood-brain barrier (BBB) endothelium. Female rats were ovariectomized (OVX) for 12-14 days, then E2 was injected at dosages of 1-100 micrograms/kg sc at 2-16 h before sampling. Glucose transport into BBB endothelial cells was assessed using 2-deoxy-[14C]glucose (2-[14C]DG) uptake. GLUT-1 protein and mRNA samples were analyzed by Western and Northern blotting, respectively. E2 treatment caused dose- and time-dependent increases in 2-[14C]DG uptake and GLUT-1 protein expression by microvessels. The peak responses were induced by 10 micrograms/kg E2 dose at the 4-h sampling time (36.0 and 31.3% increases, P < 0.05, respectively). GLUT-1 mRNA demonstrated a transient increase at 15 min (55%, P < 0.05), then decreased to basal level by 2 h. This study shows that in vivo treatment with E2 increases 2-[14C]DG uptake into the BBB endothelial cells and suggests this E2 effect is due to its modulation of GLUT-1 mRNA and protein.


This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
T. P. Obrenovitch
Molecular Physiology of Preconditioning-Induced Brain Tolerance to Ischemia
Physiol Rev, January 1, 2008; 88(1): 211 - 247.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. N. Krause, S. P. Duckles, and D. A. Pelligrino
Influence of sex steroid hormones on cerebrovascular function
J Appl Physiol, October 1, 2006; 101(4): 1252 - 1261.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
C. M. CHENG, M. COHEN, J. WANG, and C. A. BONDY
Estrogen augments glucose transporter and IGF1 expression in primate cerebral cortex
FASEB J, April 1, 2001; 15(6): 907 - 915.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online