| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Endocrinology and Metabolism, Vol 272, Issue 2 E312-E319, Copyright © 1997 by American Physiological Society
ARTICLES |
J. M. Martins, W. A. Banks and A. J. Kastin
Veterans Affairs Medical Center and Tulane University School of Medicine, New Orleans, Louisiana 70146, USA.
The unidirectional brain-to-blood transport system for corticotropin-releasing hormone (CRH) across the blood-brain barrier could be instrumental in the homeostasis of central CRH. To characterize this system, the intracerebroventricular injection of 125I-CRH was used in mice. CRH was rapidly transported out of the brain with a half-time disappearance (t1/2) of 15 min, much faster than albumin (t1/2 = 50 min). Kinetic analysis revealed a saturable component with a low maximum velocity (apaproximately 0.020 nmol x min(-1) x brain(-1)) and low capacity (Michaelis constant approximately 1.4 nmol/brain). Transport was inhibited by verapamil, ouabain, and colchicine but not by cyclosporin. Transport was increased by corticosterone and inhibited by tumor necrosis factor-alpha and beta-endorphin. These results suggest that the specific unidirectional brain-to-blood transport system for CRH is dependent on energy and calcium channels, involves microtubules, is independent of the P-glycoprotein transporter, and is acutely modulated by adrenal steroids, cytokines, and endogenous opiates. This suggests its participation in the control of the stress response.
This article has been cited by other articles:
|
|
 |
|
  R. Bisschops, P. Vanden Berghe, G. Sarnelli, J. Janssens, and J. Tack CRF-induced calcium signaling in guinea pig small intestine myenteric neurons involves CRF-1 receptors and activation of voltage-sensitive calcium channels Am J Physiol Gastrointest Liver Physiol, June 1, 2006; 290(6): G1252 - G1260. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Wang, V. Martinez, J. E. Rivier, and Y. Tache Peripheral urocortin inhibits gastric emptying and food intake in mice: differential role of CRF receptor 2 Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2001; 281(5): R1401 - R1410. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Kastin and V. Akerstrom Mahogany (1377-1428) Enters Brain by a Saturable Transport System J. Pharmacol. Exp. Ther., August 1, 2000; 294(2): 633 - 636. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. C. Bittencourt and P. E. Sawchenko Do Centrally Administered Neuropeptides Access Cognate Receptors?: An Analysis in the Central Corticotropin-Releasing Factor System J. Neurosci., February 1, 2000; 20(3): 1142 - 1156. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nozu, V. Martinez, J. Rivier, and Y. Tache Peripheral urocortin delays gastric emptying: role of CRF receptor 2 Am J Physiol Gastrointest Liver Physiol, April 1, 1999; 276(4): G867 - G874. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Martinez, E. Barquist, J. Rivier, and Y. Tache Central CRF inhibits gastric emptying of a nutrient solid meal in rats: the role of CRF2 receptors Am J Physiol Gastrointest Liver Physiol, May 1, 1998; 274(5): G965 - G970. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Martins, W. A. Banks, and A. J. Kastin Transport of CRH from mouse brain directly affects peripheral production of beta -endorphin by the spleen Am J Physiol Endocrinol Metab, December 1, 1997; 273(6): E1083 - E1089. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Million, C. Maillot, P. Saunders, J. Rivier, W. Vale, and Y. Tache Human urocortin II, a new CRF-related peptide, displays selective CRF2-mediated action on gastric transit in rats Am J Physiol Gastrointest Liver Physiol, January 1, 2002; 282(1): G34 - G40. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
