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Am J Physiol Endocrinol Metab 271: E529-E534, 1996;
0193-1849/96 $5.00
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AJP - Endocrinology and Metabolism, Vol 271, Issue 3 E529-E534, Copyright © 1996 by American Physiological Society

ARTICLES

Acetaminophen glucuronidation accurately reflects gluconeogenesis in fasted dogs

W. F. Schwenk and J. C. Kahl
Department of Pediatrics, Mayo Clinic, Rochester, Minnesota 55905, USA.

To assess whether acetaminophen glucuronide accurately reflects uridyl diphosphate-glucose (UDP-glucose) derived from gluconeogenesis during fasting, three mongrel dogs received infusions of [U-14C]lactate, [1-13C]galactose, and [6-3H]glucose (after fasting overnight or for 2.5 days). After initiation of the isotopes (3 h), acetaminophen was given, and the urinary acetaminophen glucuronide was isolated. The mean plasma [14C]glucose specific activity (SA) was similar to the mean urinary acetaminophen glucuronide SA both after fasting overnight [299 +/- 19 vs. 296 +/- 14 disintegrations.min-1 (dpm).mumol-1, respectively] and after 2.5 days of fasting (511 +/- 8 vs. 562 +/- 32 dpm/mumol, respectively). Mean plasma glucose flux calculated using [6-3H]glucose decreased (P < 0.05) with two additional days of fasting (18.7 +/- 1.2 vs. 13.6 +/- 0.6 mumol.kg-1.min-1), as did intrahepatic (P < 0.05) UDP-glucose flux measured using [1-13C]galactose (8.6 +/- 0.7 vs. 5.5 +/- 0.3 mumol.kg-1.min-1). We conclude that, in fasted dogs, plasma glucose and UDP-glucose, as sampled by acetaminophen, equally reflect gluconeogenesis and appear to come from the same pool of glucose 6-phosphate. In addition, cycling of glucose moieties through UDP-glucose and glycogen decreases with an increased period of fasting.


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V. Chandramouli, K. Ekberg, W. C. Schumann, S. C. Kalhan, J. Wahren, and B. R. Landau
Quantifying gluconeogenesis during fasting
Am J Physiol Endocrinol Metab, December 1, 1997; 273(6): E1209 - E1215.
[Abstract] [Full Text] [PDF]


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