AJP - Endocrinology and Metabolism, Vol 271, Issue 3 E477-E484, Copyright © 1996 by American Physiological Society

ARTICLES

Chronic ethanol feeding in a high-fat diet decreases insulin-stimulated glucose transport in rat adipocytes

J. J. Wilkes, L. L. DeForrest and L. E. Nagy
Department of Human Biology and Nutritional Sciences, University of Guelph, Ontario, Canada.

Ethanol consumption has been associated with glucose intolerance and insulin resistance and is suggested to be an independent risk factor in the development of non-insulin-dependent diabetes mellitus. We have investigated the long-term effects of ethanol consumption on insulin-regulated glucose transport in rat adipocytes. Male Wistar rats were fed a high-fat liquid diet containing 35% ethanol (ethanol fed) or a control diet that isocalorically substituted maltose dextrin for ethanol (ad libitum). A third group was pair fed the control diet. Basal rates of 2-deoxyglucose uptake were similar in adipocytes from all three groups. Treatment with insulin increased 2-deoxyglucose uptake in ad libitum- and pair-fed rats but did not stimulate uptake in ethanol-fed rats. Similarly, although okadaic acid increased 2-deoxyglucose uptake in pair-fed rats, it had no effect in ethanol-fed rats. GLUT-1 quantity was greater in pair-fed and ethanol-fed rats compared with ad libitum controls. GLUT-4 was decreased in ethanol-fed compared with pair-fed rats but was not different from ad libitum controls. In ad libitum- and pair-fed rats, insulin increased the translocation of GLUT-4 to the cell surface by 2.0-fold. In contrast, translocation of GLUT-4 was not observed after insulin stimulation of ethanol-fed rats, paralleling the loss of insulin-stimulated glucose uptake. In ethanol-fed rats, GLUT-4 protein quantity was negatively associated with increased Gs alpha protein and isoproterenol-stimulated adenosine 3',5'-cyclic monophosphate production. These data suggest that loss of insulin-stimulated glucose uptake in rat adipocytes after chronic ethanol feeding is at least partially due to decreased movement of GLUT-4 to the cell surface after insulin stimulation.

This article has been cited by other articles:

				X. Chen, B. M. Sebastian, and L. E. Nagy Chronic ethanol feeding to rats decreases adiponectin secretion by subcutaneous adipocytes Am J Physiol Endocrinol Metab, February 1, 2007; 292(2): E621 - E628. [Abstract] [Full Text] [PDF]

				B. M. Sebastian and L. E. Nagy Decreased insulin-dependent glucose transport by chronic ethanol feeding is associated with dysregulation of the Cbl/TC10 pathway in rat adipocytes Am J Physiol Endocrinol Metab, December 1, 2005; 289(6): E1077 - E1084. [Abstract] [Full Text] [PDF]

				N. Rachdaoui, B. M. Sebastian, and L. E. Nagy Chronic ethanol feeding impairs endothelin-1-stimulated glucose uptake via decreased G{alpha}11 expression in rat adipocytes Am J Physiol Endocrinol Metab, August 1, 2003; 285(2): E303 - E310. [Abstract] [Full Text] [PDF]

				B. Yu, A. Schroeder, and L. E. Nagy Ethanol stimulates glucose uptake and translocation of GLUT-4 in H9c2 myotubes via a Ca2+-dependent mechanism Am J Physiol Endocrinol Metab, December 1, 2000; 279(6): E1358 - E1365. [Abstract] [Full Text] [PDF]

				J. J. Wilkes and A. Bonen Reduced insulin-stimulated glucose transport in denervated muscle is associated with impaired Akt-alpha activation Am J Physiol Endocrinol Metab, October 1, 2000; 279(4): E912 - E919. [Abstract] [Full Text] [PDF]

				F. M. Carver, I. A. Shibley Jr., D. S. Miles, J. S. Pennington, and S. N. Pennington Increased intracellular localization of brain GLUT-1 transporter in response to ethanol during chick embryogenesis Am J Physiol Endocrinol Metab, October 1, 1999; 277(4): E750 - E759. [Abstract] [Full Text] [PDF]

				A. Aldred and L. E. Nagy Ethanol dissociates hormone-stimulated cAMP production from inhibition of TNF-alpha production in rat Kupffer cells Am J Physiol Gastrointest Liver Physiol, January 1, 1999; 276(1): G98 - G106. [Abstract] [Full Text] [PDF]

				J. J. Wilkes, A. Bonen, and R. C. Bell A modified high-fat diet induces insulin resistance in rat skeletal muscle but not adipocytes Am J Physiol Endocrinol Metab, October 1, 1998; 275(4): E679 - E686. [Abstract] [Full Text] [PDF]

				R. Kishore, J. R. Hill, M. R. McMullen, J. Frenkel, and L. E. Nagy ERK1/2 and Egr-1 contribute to increased TNF-alpha production in rat Kupffer cells after chronic ethanol feeding Am J Physiol Gastrointest Liver Physiol, January 1, 2002; 282(1): G6 - G15. [Abstract] [Full Text] [PDF]