AJP - Endocrinology and Metabolism, Vol 271, Issue 1 E58-E64, Copyright © 1996 by American Physiological Society

ARTICLES

Kinetic aspects of acetate metabolism in healthy humans using [1-13C] acetate

E. Pouteau, H. Piloquet, P. Maugeais, M. Champ, H. Dumon, P. Nguyen and M. Krempf
Laboratory of Human Nutrition and of Animal Nutrition, Veterinary School, Nantes, France.

Acetate metabolism in humans is not well known. Kinetic aspects of acetate were investigated in the postabsorptive state on healthy subjects. In a first study, six subjects were infused with a primed constant infusion of [1-13C]acetate for 3 h and a prime of NaH13CO3. No difference was found between arterialized and venous tracer enrichments from the arm, although arterialized acetate concentrations were higher (74 +/- 12 vs. 59 +/- 14 mumol/l, P < 0.05), suggesting that the hand muscles used but did not produce acetate in the postabsorptive state. Total body flux of acetate was 8.4 +/- 0.6 mumol.kg-1.min-1, of which 69 +/- 5% was oxidized. Acetate contributed to 6.5 +/- 0.4% of the basal energy expenditure. In a second study, five volunteers were submitted to a gastric infusion for 3 h followed by an intravenous infusion of [1-13C]acetate for 3 h. Higher fluxes were observed with the tracer gastric infusion, and the first-pass removal of acetate within the splanchnic bed was 60 +/- 7%. Acetate contributes significantly to the energy supply of the body. It is mainly used by the liver when produced in the gut.

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