AJP - Endo Add DOIs to your references at manuscript stage!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 270: E882-E889, 1996;
0193-1849/96 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yang, D.
Right arrow Articles by Brunengraber, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yang, D.
Right arrow Articles by Brunengraber, H.

AJP - Endocrinology and Metabolism, Vol 270, Issue 5 E882-E889, Copyright © 1996 by American Physiological Society

ARTICLES

Noninvasive probing of citric acid cycle intermediates in primate liver with phenylacetylglutamine

D. Yang, S. F. Previs, C. A. Fernandez, S. Dugelay, M. V. Soloviev, J. W. Hazey, K. C. Agarwal, W. C. Levine, F. David, P. Rinaldo, M. Beylot and H. Brunengraber
Department of Nutrition, Case Western Reserve University, Cleveland, Ohio 44106, USA.

In human and primate liver, phenylacetate and glutamine form phenylacetylglutamine, which is excreted in urine. Probing noninvasively the labeling pattern of liver citric acid cycle intermediates with phenylacetylglutamine assumes that the labeling pattern of its glutamine moiety reflects that of liver alpha-ketoglutarate. To validate this probe, we infused monkeys with [U-13C3]lactate, [3-13C]lactate, [1, 2-13C2]acetate, [2-13C]acetate, [U-13C3]glycerol, or 2-[3-13C]ketoisocaproate and compared the labeling patterns of urinary phenylacetyl-glutamine with those of glutamate and glutamine in liver, plasma, muscle, and kidney and liver alpha-ketoglutarate. Only with [U-13C3]lactate or [3-13C]lactate does the labeling pattern of phenylacetylglutamine reflect patterns of liver alpha-ketoglutarate and glutamate. With [13C]acetate, muscle and kidney glutamate are more labeled than liver metabolites. This confirms that with [13C]acetate, the labeling pattern of liver metabolites is influenced by 13CO2 and [13C]glutamine made in peripheral tissues. Our data validate the use of phenylacetylglutamine labeled from [3-13C]lactate or [3-13C]pyruvate to probe noninvasively the pyruvate carboxylase-to-pyruvate dehydrogenase flux ratio in human subjects.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
A. Conjard, S. Dugelay, M.-F. Chauvin, D. Durozard, G. Baverel, and G. Martin
The Anaplerotic Substrate Alanine Stimulates Acetate Incorporation into Glutamate and Glutamine in Rabbit Kidney Tubules. A 13C NMR STUDY
J. Biol. Chem., August 9, 2002; 277(33): 29444 - 29454.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
D. Yang and H. Brunengraber
Glutamate, a Window on Liver Intermediary Metabolism
J. Nutr., April 1, 2000; 130(4): 991 - 991.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
F. Diraison, V. Large, C. Maugeais, M. Krempf, and M. Beylot
Noninvasive tracing of human liver metabolism: comparison of phenylacetate and apoB-100 to sample glutamine
Am J Physiol Endocrinol Metab, September 1, 1999; 277(3): E529 - E536.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. G. Jones, M. A. Solomon, A. D. Sherry, F. M. H. Jeffrey, and C. R. Malloy
13C NMR measurements of human gluconeogenic fluxes after ingestion of [U-13C]propionate, phenylacetate, and acetaminophen
Am J Physiol Endocrinol Metab, November 1, 1998; 275(5): E843 - E852.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online