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AJP - Endocrinology and Metabolism, Vol 270, Issue 1 E43-E50, Copyright © 1996 by American Physiological Society
ARTICLES |
L. Voisin, K. Gray, K. M. Flowers, S. R. Kimball, L. S. Jefferson and T. C. Vary
Department of Cellular and Molecular Physiology, Pennsylvania State University College of Medicine, Hershey 17033, USA.
Sepsis causes an inhibition of protein synthesis in skeletal muscles composed of fast-twitch fibers, in part, as a result of a decreased activity of the eukaryotic initiation factor 2B (eIF-2B). In the present study, we investigated the expression of two subunits of eIF-2B, i.e., the beta- and epsilon-subunits during sepsis. The expression of both beta- and epsilon-subunits of eIF-2B in gastrocnemius was decreased approximately 50% from control values during the first 5 days after induction of sepsis. The decreased expression of eIF-2B epsilon during sepsis correlated with similar reductions in eIF-2B epsilon mRNA. Restoration of protein synthesis (10 days postsurgery) was associated with a return of eIF-2B epsilon expression to values observed in control rats. Expression of eIF-2B epsilon was not altered in heart during sepsis or in gastrocnemius from nonseptic abscess animals. Amrinone, which ameliorated the inhibition of protein synthesis during sepsis, also prevented the fall in eIF-2B epsilon protein after 5 days of infection. The data provide evidence that expression of eIF-2B epsilon is markedly influenced in gastrocnemius during the course of the septic episode and support the concept that this change is a mechanism responsible for the inhibition of protein synthesis observed under this condition.
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T. C. Vary IGF-I stimulates protein synthesis in skeletal muscle through multiple signaling pathways during sepsis Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2006; 290(2): R313 - R321. [Abstract] [Full Text] [PDF]
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T. C. Vary, G. Deiter, and S. R. Kimball Phosphorylation of eukaryotic initiation factor eIF2Bepsilon in skeletal muscle during sepsis Am J Physiol Endocrinol Metab, November 1, 2002; 283(5): E1032 - E1039. [Abstract] [Full Text] [PDF]
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T. C. Vary, L. S. Jefferson, and S. R. Kimball Insulin fails to stimulate muscle protein synthesis in sepsis despite unimpaired signaling to 4E-BP1 and S6K1 Am J Physiol Endocrinol Metab, November 1, 2001; 281(5): E1045 - E1053. [Abstract] [Full Text] [PDF]
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C. H. Lang, R. A. Frost, V. Kumar, and T. C. Vary Impaired myocardial protein synthesis induced by acute alcohol intoxication is associated with changes in eIF4F Am J Physiol Endocrinol Metab, November 1, 2000; 279(5): E1029 - E1038. [Abstract] [Full Text] [PDF]
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E. Svanberg, R. A. Frost, C. H. Lang, J. Isgaard, L. S. Jefferson, S. R. Kimball, and T. C. Vary IGF-I/IGFBP-3 binary complex modulates sepsis-induced inhibition of protein synthesis in skeletal muscle Am J Physiol Endocrinol Metab, November 1, 2000; 279(5): E1145 - E1158. [Abstract] [Full Text] [PDF]
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T. C. Vary and S. R. Kimball Effect of sepsis on eIE4E availability in skeletal muscle Am J Physiol Endocrinol Metab, November 1, 2000; 279(5): E1178 - E1184. [Abstract] [Full Text] [PDF]
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C. H. Lang, R. A. Frost, L. S. Jefferson, S. R. Kimball, and T. C. Vary Endotoxin-induced decrease in muscle protein synthesis is associated with changes in eIF2B, eIF4E, and IGF-I Am J Physiol Endocrinol Metab, June 1, 2000; 278(6): E1133 - E1143. [Abstract] [Full Text] [PDF]
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 P. A. Farrell, J. M. Hernandez, M. J. Fedele, T. C. Vary, S. R. Kimball, and L. S. Jefferson Eukaryotic initiation factors and protein synthesis after resistance exercise in rats J Appl Physiol, March 1, 2000; 88(3): 1036 - 1042. [Abstract] [Full Text] [PDF]
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C. H. Lang, D. Wu, R. A. Frost, L. S. Jefferson, T. C. Vary, and S. R. Kimball Chronic alcohol feeding impairs hepatic translation initiation by modulating eIF2 and eIF4E Am J Physiol Endocrinol Metab, November 1, 1999; 277(5): E805 - E814. [Abstract] [Full Text] [PDF]
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C. H. Lang, D. Wu, R. A. Frost, L. S. Jefferson, S. R. Kimball, and T. C. Vary Inhibition of muscle protein synthesis by alcohol is associated with modulation of eIF2B and eIF4E Am J Physiol Endocrinol Metab, August 1, 1999; 277(2): E268 - E276. [Abstract] [Full Text] [PDF]
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R. Cooney, S. R. Kimball, R. Eckman, G. Maish III, M. Shumate, and T. C. Vary TNF-binding protein ameliorates inhibition of skeletal muscle protein synthesis during sepsis Am J Physiol Endocrinol Metab, April 1, 1999; 276(4): E611 - E619. [Abstract] [Full Text] [PDF]
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C. H. Lang, R. A. Frost, A. C. Nairn, D. A. MacLean, and T. C. Vary TNF-alpha impairs heart and skeletal muscle protein synthesis by altering translation initiation Am J Physiol Endocrinol Metab, February 1, 2002; 282(2): E336 - E347. [Abstract] [Full Text] [PDF]
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