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Am J Physiol Endocrinol Metab 269: E969-E976, 1995;
0193-1849/95 $5.00
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AJP - Endocrinology and Metabolism, Vol 269, Issue 5 E969-E976, Copyright © 1995 by American Physiological Society

ARTICLES

Recording of a large-conductance chloride channel in normal rat lactotrophs

M. Fahmi, L. Garcia, A. Taupignon, B. Dufy and P. Sartor
Laboratoire de Neurophysiologie, Centre National de la Recherche Scientifique Unite de Recherche Associee 1200, Universite de Bordeaux 2, France.

Membrane current fluctuations resembling channel openings and closings were observed in the whole cell configuration of the patch-clamp technique in normal rat lactotrophs in primary culture. Using high-gain head stage in whole cell configuration, we characterized the nature and pharmacological properties of the ionic channel underlying these fluctuations. This channel, found in small numbers (< 10 per cell), was specific for Cl- because its reversal potential varied with Cl- gradients, according to the Nernst equation, and its unitary amplitude was linearly related to membrane potential from -100 to 0 mV. Slope conductance was close to 100 pS. Analyzing open times, we demonstrated its Ca2+ and potential dependence. Four sublevels were observed. We suggest that this channel, belonging to the background Cl- channel group, takes part in the regulation of intracellular Cl- concentration of normal rat lactotrophs.


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