AJP - Endocrinology and Metabolism, Vol 268, Issue 4 E722-E729, Copyright © 1995 by American Physiological Society

ARTICLES

Modulation of in vivo insulin action by dietary protein during pregnancy

M. C. Sugden and M. J. Holness
Department of Biochemistry, Queen Mary and Westfield College, University of London, United Kingdom.

Rats were provided with a standard 20% protein diet or an isocaloric 8% protein diet from day 1 of gestation and were studied on day 19 of pregnancy. Fetal numbers per dam were unchanged, but total fetal weight at day 19 of gestation was reduced by 10% (P < 0.001) in the 8% protein group. In the basal state, endogenous glucose production (Ra) and muscle glucose uptake/phosphorylation were not significantly affected by dietary protein content. The glucose infusion rate required to maintain glycemia and Ra during euglycemic-hyperinsulinemic clamp (insulin infusion rate of 4.17 mU.kg-1.min-1) were reduced in the 8% protein group by 17% (P < 0.05) and 76% (P < 0.001), respectively. Suppression of Ra by insulin was not significant in the 20% protein group. Insulin-stimulated glucose disappearance (Rd) was 24% lower (P < 0.001) in the 8% protein group (25.0 +/- 0.8 mg.min-1.kg-1) than in the 20% protein group (32.7 +/- 0.5 mg.min-1.kg-1). The overall increment in muscle glucose utilization index (mean of 6 muscles) elicited by insulin was impaired by 38.1 +/- 4.0%. Insulin suppressed nonesterified fatty acid concentration (NEFA) by 83% (P < 0.001) and plasma triacylglycerol concentration (TAG) by 67% (P < 0.05) in the 20% protein group but evoked only a 43% (P < 0.01) decline in plasma NEFA and did not significantly suppress plasma TAG in the 8% protein group.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				M. J. Holness and M. C. Sugden Antecedent protein restriction exacerbates development of impaired insulin action after high-fat feeding Am J Physiol Endocrinol Metab, January 1, 1999; 276(1): E85 - E93. [Abstract] [Full Text] [PDF]