AJP - Endo AJP: Heart and Circulatory Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 268: E712-E721, 1995;
0193-1849/95 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Youn, J. H.
Right arrow Articles by Steil, G. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Youn, J. H.
Right arrow Articles by Steil, G. M.

AJP - Endocrinology and Metabolism, Vol 268, Issue 4 E712-E721, Copyright © 1995 by American Physiological Society

ARTICLES

Assessment of extracellular glucose distribution and glucose transport activity in conscious rats

J. H. Youn, J. K. Kim and G. M. Steil
Department of Physiology and Biophysics, University of Southern California School of Medicine, Los Angeles 90033, USA.

The effects of insulin on extracellular glucose distribution and cellular glucose transport activity were studied by simultaneously analyzing the plasma kinetics of L-[1-14C]glucose and 3-O-[3H]methylglucose after an intravenous injection during saline or insulin infusion (euglycemic glucose clamp) in conscious rats (n = 7 for each). The time profiles of plasma L-glucose were almost superimposable in the two protocols, and compartmental analysis showed that neither distribution volumes nor distribution rate constants were affected with insulin (P > 0.05 for all), suggesting that glucose distribution within the extracellular space was not influenced with insulin. In contrast, the time profile of plasma 3-O-methylglucose (3-MG) was markedly altered with insulin; the initial decrease was much faster during insulin infusion than during saline infusion, indicating stimulation of 3-MG transport into intracellular spaces with insulin. The 3-MG data were analyzed using a comprehensive model separately describing extracellular distribution and cellular transport of 3-MG by incorporating information on extracellular distribution kinetics obtained from L-glucose data. The combined L-glucose and 3-MG kinetic analysis precisely estimated insulin's effect in vivo to stimulate glucose transport into and out of intracellular spaces. We conclude that 1) insulin does not affect extracellular glucose distribution kinetics or volumes in conscious rats and 2) insulin's effects on cellular glucose transport in vivo can be assessed by simultaneous analysis of plasma L-glucose and 3-MG kinetics.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
M. S. Rhee, A. Perianayagam, P. Chen, J. H. Youn, and A. A. McDonough
Dexamethasone treatment causes resistance to insulin-stimulated cellular potassium uptake in the rat
Am J Physiol Cell Physiol, November 1, 2004; 287(5): C1229 - C1237.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
H. A. Petersen, P. T. Fueger, D. P. Bracy, D. H. Wasserman, and A. E. Halseth
Fiber type-specific determinants of Vmax for insulin-stimulated muscle glucose uptake in vivo
Am J Physiol Endocrinol Metab, March 1, 2003; 284(3): E541 - E548.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
C. S. Choi, F. N. Lee, A. A. McDonough, and J. H. Youn
Independent Regulation of In Vivo Insulin Action on Glucose Versus K+ Uptake by Dietary Fat and K+ Content
Diabetes, April 1, 2002; 51(4): 915 - 920.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
C. S. Choi, C. B. Thompson, P. K. K. Leong, A. A. McDonough, and J. H. Youn
Short-term K+ deprivation provokes insulin resistance of cellular K+ uptake revealed with the K+ clamp
Am J Physiol Renal Physiol, January 1, 2001; 280(1): F95 - F102.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
L. D. Klaman, O. Boss, O. D. Peroni, J. K. Kim, J. L. Martino, J. M. Zabolotny, N. Moghal, M. Lubkin, Y.-B. Kim, A. H. Sharpe, et al.
Increased Energy Expenditure, Decreased Adiposity, and Tissue-Specific Insulin Sensitivity in Protein-Tyrosine Phosphatase 1B-Deficient Mice
Mol. Cell. Biol., August 1, 2000; 20(15): 5479 - 5489.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
K. Zierler
Whole body glucose metabolism
Am J Physiol Endocrinol Metab, March 1, 1999; 276(3): E409 - E426.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
R. M. O'Doherty, A. E. Halseth, D. K. Granner, D. P. Bracy, and D. H. Wasserman
Analysis of insulin-stimulated skeletal muscle glucose uptake in conscious rat using isotopic glucose analogs
Am J Physiol Endocrinol Metab, February 1, 1998; 274(2): E287 - E296.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
G. W. Cline, B. M. Jucker, Z. Trajanoski, A. J. M. Rennings, and G. I. Shulman
A novel 13C NMR method to assess intracellular glucose concentration in muscle, in vivo
Am J Physiol Endocrinol Metab, February 1, 1998; 274(2): E381 - E389.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
G. A. Dienel, N. F. Cruz, K. Adachi, L. Sokoloff, and J. E. Holden
Determination of local brain glucose level with [14C]methylglucose: effects of glucose supply and demand
Am J Physiol Endocrinol Metab, November 1, 1997; 273(5): E839 - E849.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online