AJP - Endocrinology and Metabolism, Vol 268, Issue 3 E491-E500, Copyright © 1995 by American Physiological Society

ARTICLES

Amrinone prevents muscle protein wasting during chronic sepsis

C. V. Jurasinski, L. Kilpatrick and T. C. Vary
Department of Cellular and Molecular Physiology, Penn State University College of Medicine, Hershey 17033.

The time course for the effects of sepsis on rates of protein synthesis, RNA contents, and translational efficiencies was measured in mixed muscles of rat hindlimb perfused in vitro 3, 5, and 10 days after induction of sepsis. Furthermore, the effect of daily injections of amrinone (5 mg.kg-1.day-1) on muscle protein synthesis was investigated. On day 3 of sepsis, decreased rates of protein synthesis in muscle from untreated septic animals or septic rats treated with amrinone resulted from a reduced food intake. When food intake became normalized to control after 5 days, rates of protein synthesis in untreated septic rats remained depressed. Treatment of septic animals with amrinone for 5 days prevented the sepsis-induced inhibition of protein synthesis by abolishing the inhibition of peptide-chain initiation and restoring translational efficiency to control values. In contrast, amrinone treatment of control rats for 5 days did not cause an accretion of muscle protein or augment protein synthesis. Ten days after induction of sepsis, there were no differences in rates of protein synthesis, RNA content, or translational efficiency in septic animals compared with control or amrinone-treated septic rats. Thus, amrinone prevented the sepsis-induced abnormalities in skeletal muscle protein synthesis.

This article has been cited by other articles:

				T. C. Vary Acute Oral Leucine Administration Stimulates Protein Synthesis during Chronic Sepsis through Enhanced Association of Eukaryotic Initiation Factor 4G with Eukaryotic Initiation Factor 4E in Rats J. Nutr., September 1, 2007; 137(9): 2074 - 2079. [Abstract] [Full Text] [PDF]

				A. M. Baviera, N. M. Zanon, L. C. Carvalho Navegantes, R. H. Migliorini, and I. d. C. Kettelhut Pentoxifylline inhibits Ca2+-dependent and ATP proteasome-dependent proteolysis in skeletal muscle from acutely diabetic rats Am J Physiol Endocrinol Metab, March 1, 2007; 292(3): E702 - E708. [Abstract] [Full Text] [PDF]

				T. C. Vary IGF-I stimulates protein synthesis in skeletal muscle through multiple signaling pathways during sepsis Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2006; 290(2): R313 - R321. [Abstract] [Full Text] [PDF]

				T. C. Vary, G. Deiter, and S. R. Kimball Phosphorylation of eukaryotic initiation factor eIF2Bepsilon in skeletal muscle during sepsis Am J Physiol Endocrinol Metab, November 1, 2002; 283(5): E1032 - E1039. [Abstract] [Full Text] [PDF]

				E. F. M. Wouters, E. C. Creutzberg, and A. M. W. J. Schols Systemic Effects in COPD* Chest, May 1, 2002; 121 (2009): 127S - 130S. [Abstract] [Full Text] [PDF]

				C. H. Lang, G. J. Nystrom, and R. A. Frost Burn-induced changes in IGF-I and IGF-binding proteins are partially glucocorticoid dependent Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2002; 282(1): R207 - R215. [Abstract] [Full Text] [PDF]

				T. C. Vary, L. S. Jefferson, and S. R. Kimball Insulin fails to stimulate muscle protein synthesis in sepsis despite unimpaired signaling to 4E-BP1 and S6K1 Am J Physiol Endocrinol Metab, November 1, 2001; 281(5): E1045 - E1053. [Abstract] [Full Text] [PDF]

				E. Svanberg, R. A. Frost, C. H. Lang, J. Isgaard, L. S. Jefferson, S. R. Kimball, and T. C. Vary IGF-I/IGFBP-3 binary complex modulates sepsis-induced inhibition of protein synthesis in skeletal muscle Am J Physiol Endocrinol Metab, November 1, 2000; 279(5): E1145 - E1158. [Abstract] [Full Text] [PDF]

				T. C. Vary and S. R. Kimball Effect of sepsis on eIE4E availability in skeletal muscle Am J Physiol Endocrinol Metab, November 1, 2000; 279(5): E1178 - E1184. [Abstract] [Full Text] [PDF]

				T. C. Vary, L. S. Jefferson, and S. R. Kimball Role of eIF4E in stimulation of protein synthesis by IGF-I in perfused rat skeletal muscle Am J Physiol Endocrinol Metab, January 1, 2000; 278(1): E58 - E64. [Abstract] [Full Text] [PDF]

				R. Cooney, S. R. Kimball, R. Eckman, G. Maish III, M. Shumate, and T. C. Vary TNF-binding protein ameliorates inhibition of skeletal muscle protein synthesis during sepsis Am J Physiol Endocrinol Metab, April 1, 1999; 276(4): E611 - E619. [Abstract] [Full Text] [PDF]

				D. Breuille, L. Voisin, M. Contrepois, M. Arnal, F. Rose, and C. Obled A Sustained Rat Model for Studying the Long-Lasting Catabolic State of Sepsis Infect. Immun., March 1, 1999; 67(3): 1079 - 1085. [Abstract] [Full Text] [PDF]

				L. Voisin, D. Breuille, B. Ruot, C. Ralliere, F. Rambourdin, M. Dalle, and C. Obled Cytokine modulation by PX differently affects specific acute phase proteins during sepsis in rats Am J Physiol Regulatory Integrative Comp Physiol, November 1, 1998; 275(5): R1412 - R1419. [Abstract] [Full Text] [PDF]

				T. C. Vary, D. Dardevet, J. Grizard, L. Voisin, C. Buffiere, P. Denis, D. Breuille, and C. Obled Differential regulation of skeletal muscle protein turnover by insulin and IGF-I after bacteremia Am J Physiol Endocrinol Metab, October 1, 1998; 275(4): E584 - E593. [Abstract] [Full Text] [PDF]

				C. H. Lang, R. A. Frost, A. C. Nairn, D. A. MacLean, and T. C. Vary TNF-alpha impairs heart and skeletal muscle protein synthesis by altering translation initiation Am J Physiol Endocrinol Metab, February 1, 2002; 282(2): E336 - E347. [Abstract] [Full Text] [PDF]