AJP - Endocrinology and Metabolism, Vol 268, Issue 2 E343-E348, Copyright © 1995 by American Physiological Society
Somatostatin receptor-effector system in rat pancreatic acinar membranes after subtotal enterectomy
I. Alvaro-Alonso, B. Colas, J. P. Esteve, C. Susini and E. Arilla
Departamento de Bioquimica y Biologia Molecular, Facultad de Medicina, Universidad de Alcala, Madrid, Spain.
In the present study we found that exocrine pancreatic hyperplasia observed
after proximal small bowel resection is accompanied by an increase in
pancreatic somatostatin (SS) content at 1 mo and an increase in the number
of SS receptors at 2 wk and 1 mo after intestinal surgery. At 6 mo after
small bowel resection SS content and SS receptors had returned to control
values. However, the original increase in SS receptor number was
accompanied by a decrease in the ability of SS to inhibit
forskolin-stimulated adenylyl cyclase (AC) activity. In addition, the
ability of 5'-guanylylimidodiphosphate (a nonhydrolyzable GTP analogue) to
inhibit SS receptor binding was decreased in pancreatic acinar membranes
from enterectomized rats at 2 wk and 1 mo after jejunoileal resection.
These data suggest that there is an abnormality in the integrity of SS
receptor binding site-G protein interactions and would explain the
decreased inactivation of AC by SS at 2 wk and 1 mo after proximal small
bowel resection.
