AJP - Endocrinology and Metabolism, Vol 267, Issue 6 E975-E982, Copyright © 1994 by American Physiological Society
Effects of a fish oil-lard diet on rat plasma lipoproteins, liver FAS, and lipolytic enzymes
F. Benhizia, I. Hainault, C. Serougne, D. Lagrange, E. Hajduch, C. Guichard, M. I. Malewiak, A. Quignard-Boulange, M. Lavau and S. Griglio
Unite de Physiopathologie de la Nutrition, Institut National de la Sante et de la Recherche Medicale U177, Paris, France.
The effects of a fish oil concentrate on blood lipids and lipoproteins were
examined in relation to their effects on liver fatty acid synthase (FAS),
3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase, adipose tissue
lipoprotein lipase (LPL), and hepatic triglyceride lipase (H-TGL). For 15
days, 2-mo-old rats were fed a control diet (10% of calories from fat, 4%
fat by weight) or diets with 50% of calories (25% wt/wt) provided by lard,
lard and fish oil calories (35%/15%), or lard and corn oil (35%/15%). The
high-lard diet increased plasma chylomicron and liver triglycerides. The
high-lard diet greatly decreased FAS, HMG-CoA reductase, and LPL
activities; it also reduced H-TGL activity. Compared with the lard diet,
the lard-fish oil diet decreased plasma TG by drastically lowering
chylomicron (4-fold, P < 0.001) and very-low-density lipoprotein levels
(P < 0.001). It also reduced high-density lipoprotein levels. The
lard-fish oil diet prevented hepatic triglyceride accumulation and
decreased FAS activity and mass by 3.5-fold (P < 0.001) but did not
further decrease HMG-CoA reductase activity. Adipose tissue LPL activity
was 2.5-fold (P < 0.001) higher with the lard-fish oil diet than with
the lard diet, and H-TGL activity decreased significantly (-32%, P <
0.01), despite unaltered levels of H-TGL mRNA. These effects were
significant with only 10% fish oil concentrate in the lard diet. They were
not observed with the lard-corn oil diet.(ABSTRACT TRUNCATED AT 250 WORDS)
