AJP - Endocrinology and Metabolism, Vol 267, Issue 5 E636-E641, Copyright © 1994 by American Physiological Society

ARTICLES

Interleukin-1 receptor antagonist prevents sepsis-induced inhibition of protein synthesis

R. Cooney, E. Owens, C. Jurasinski, K. Gray, J. Vannice and T. Vary
Department of Surgery, Pennsylvania State University, College of Medicine, Hershey 17033.

To understand the role of interleukin-1 (IL-1) as a mediator of the sepsis-induced skeletal muscle catabolism, we investigated the effects of a specific IL-1 receptor antagonist (IL-1ra) on skeletal muscle protein metabolism in a rodent model of chronic abdominal sepsis. A constant infusion of IL-1ra (2 mg.kg-1.h-1) or saline was begun immediately after the induction of sepsis and continued for 5 days. The effect of IL-1ra on protein metabolism was examined in individual muscles (gastrocnemius, soleus, heart) containing different fiber types. Infusion of IL-1ra in control animals did not alter protein metabolism in any of the muscles examined. Muscle weight, protein content, and the rate of protein synthesis in gastrocnemius were reduced by sepsis, whereas none of these parameters were affected in soleus or heart. Infusion of IL-1ra prevented the sepsis-induced loss of muscle protein and inhibition of protein synthesis in gastrocnemius but was without effect in soleus or heart. IL-1ra infusion restored translational efficiency in the gastrocnemius of septic rats and was without effect on the RNA content. These results provide evidence for a role of IL-1 as a mediator of the sepsis-induced abnormalities in skeletal muscle protein metabolism.

This article has been cited by other articles:

				T. C. Vary IGF-I stimulates protein synthesis in skeletal muscle through multiple signaling pathways during sepsis Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2006; 290(2): R313 - R321. [Abstract] [Full Text] [PDF]

				T. C. Vary, G. Deiter, and S. R. Kimball Phosphorylation of eukaryotic initiation factor eIF2Bepsilon in skeletal muscle during sepsis Am J Physiol Endocrinol Metab, November 1, 2002; 283(5): E1032 - E1039. [Abstract] [Full Text] [PDF]

				L. Voisin, D. Breuille, B. Ruot, C. Ralliere, F. Rambourdin, M. Dalle, and C. Obled Cytokine modulation by PX differently affects specific acute phase proteins during sepsis in rats Am J Physiol Regulatory Integrative Comp Physiol, November 1, 1998; 275(5): R1412 - R1419. [Abstract] [Full Text] [PDF]