AJP - Endocrinology and Metabolism, Vol 267, Issue 4 E612-E619, Copyright © 1994 by American Physiological Society
Expression of components of the RAS during prolonged blockade at different levels in primates
N. J. Samani, F. Cumin, M. Kelly and J. M. Wood
Department of Medicine, University of Leicester, United Kingdom.
To assess the effects of inhibition of the renin-angiotensin system at
different levels on plasma concentrations of components of the system and
on renin and angiotensinogen gene expression, marmosets on a low-sodium
diet were treated for 1 wk by continuous intraperitoneal infusion with
either the renin inhibitor CGP-29287, the ACE inhibitor benazeprilat, the
angiotensin II antagonist valsartan, the renin inhibitory monoclonal
antibody R-3-36-16, or vehicle. Plasma total immunoreactive renin increased
(14- to 20-fold) after all three modes of interference. Plasma
angiotensinogen was significantly reduced in the benazeprilat- and
valsartan-treated marmosets but not in the CGP-29287-treated animals.
Plasma concentration of angiotensin II was significantly decreased in the
benazeprilat-, CGP-29287-, and R-3-36-16-treated marmosets and was
increased in the valsartan-treated marmosets. Kidney renin mRNA level
increased 8- to 15-fold in all groups. Hepatic angiotensinogen mRNA level
increased with CGP-29287 treatment but decreased with the other treatments.
Kidney angiotensinogen mRNA level was not affected by any treatment.
Different modes of inhibition of the renin-angiotensin system have
different effects on plasma components of the system and liver
angiotensinogen expression.
